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PMID: 8422996
Published · ppublish
English
Journal Article
Thiamine-repressible expression vectors pREP and pRIP for fission yeast.
Abstract
The promoter and polyadenylation signal of the thiamine-repressible gene nmt1 of Schizosaccharomyces pombe have been used to construct the pREP extrachromosomally replicating plasmids and the pRIP integrative expression plasmids. These plasmids permit thiamine-mediated control of transcription to be applied to cloned genes.
Related Genes
MeSH Terms
Base Sequence
Cloning, Molecular
DNA, Fungal
Gene Expression Regulation, Fungal
Genetic Vectors
Molecular Sequence Data
Plasmids
Restriction Mapping
Schizosaccharomyces/genetics,metabolism
Thiamine/metabolism
Chemicals
DNA, Fungal
Thiamine
Article Info
Journal
Gene
Abbr.
Gene
ISSN
0378-1119
Published
1993-01-15
Pages
127-30
Language
English
Region
Netherlands
NLM ID
7706761
Subset
IM
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