Home LiteratureArticle Details
PMID: 8423816 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Increased UV resistance of a xeroderma pigmentosum revertant cell line is correlated with selective repair of the transcribed strand of an expressed gene.

Molecular and cellular biology ·Vol. 13 ·No. 2 ·1993-02-00 ·Pages 970-6

Lommel L, Hanawalt PC

Abstract

A UV-resistant revertant (XP129) of a xeroderma pigmentosum group A cell line has been reported to be totally deficient in repair of cyclobutane pyrimidine dimers (CPDs) but proficient in repair of 6-4 photoproducts. This finding has been interpreted to mean that CPDs play no role in cell killing by UV. We have analyzed the fine structure of repair of CPDs in the dihydrofolate reductase gene in the revertant. In this essential, active gene, we observe that repair of the transcribed strand is as efficient as that in normal, repair-proficient human cells, but repair of the nontranscribed strand is not. Within 4 h after UV at 7.5 J/m2, over 50% of the CPDs were removed, and by 8 h, 80% of the CPDs were removed. In contrast, there was essentially no removal from the nontranscribed strand even by 24 h. Our results demonstrate that overall repair measurements can be misleading, and they support the hypothesis that removal of CPDs from the transcribed strands of expressed genes is essential for UV resistance.

Related Genes
DHFR
MeSH Terms
Animals Cell Line, Transformed Cricetinae Cricetulus DNA/genetics,radiation effects DNA Repair/radiation effects Deoxyribonuclease HindIII/metabolism Deoxyribonucleases, Type II Site-Specific/metabolism Humans Kinetics Pyrimidine Dimers Tetrahydrofolate Dehydrogenase/genetics,metabolism Transcription, Genetic Ultraviolet Rays Xeroderma Pigmentosum/enzymology,genetics
Chemicals
Pyrimidine Dimers DNA Tetrahydrofolate Dehydrogenase Deoxyribonuclease HindIII Deoxyribonucleases, Type II Site-Specific GGTACC-specific type II deoxyribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lommel L
Department of Biological Sciences, Stanford University, California 94305-5020.
Hanawalt P C
References (31)
31 references, click to expand
  1. Intermediate (6-4) photoproduct repair in Chinese hamster V79 mutant V-H1 correlates with intermediate levels of DNA incision and repair replication.
    Mutat Res. 1989 May;226(1):43-7 PMID: 2716768
  2. Comparison of the cleavage of pyrimidine dimers by the bacteriophage T4 and Micrococcus luteus UV-specific endonucleases.
    J Biol Chem. 1980 Dec 25;255(24):12047-50 PMID: 6254991
  3. DNA strand specificity for UV-induced mutations in mammalian cells.
    Mol Cell Biol. 1989 Mar;9(3):1277-83 PMID: 2725498
  4. CHO mutant UV61 removes (6-4) photoproducts but not cyclobutane dimers.
    Mutagenesis. 1989 Mar;4(2):140-6 PMID: 2659925
  5. Differential repair of UV damage in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1989 Jun 26;17(12):4433-9 PMID: 2664708
  6. Xeroderma pigmentosum complementation group XP-I withdrawn.
    Mutat Res. 1989 Sep;218(2):149-51 PMID: 2770764
  7. The biology of the (6-4) photoproduct.
    Photochem Photobiol. 1989 Jun;49(6):805-19 PMID: 2672059
  8. Induction of the Escherichia coli lactose operon selectively increases repair of its transcribed DNA strand.
    Nature. 1989 Nov 2;342(6245):95-8 PMID: 2554145
  9. Cyclobutane-pyrimidine dimer excision in UV-sensitive CHO mutants and the effect of the human ERCC2 repair gene.
    Mutat Res. 1990 May;235(3):157-63 PMID: 2342503
  10. Site-specific DNA repair at the nucleosome level in a yeast minichromosome.
    Cell. 1990 May 18;61(4):675-84 PMID: 2188732
  11. Analysis of a human DNA excision repair gene involved in group A xeroderma pigmentosum and containing a zinc-finger domain.
    Nature. 1990 Nov 1;348(6296):73-6 PMID: 2234061
  12. Molecular analysis of ultraviolet-induced mutations in a xeroderma pigmentosum cell line.
    J Mol Biol. 1991 Jan 20;217(2):217-22 PMID: 1992158
  13. Cell cycle-dependent strand bias for UV-induced mutations in the transcribed strand of excision repair-proficient human fibroblasts but not in repair-deficient cells.
    Mol Cell Biol. 1991 Apr;11(4):1927-34 PMID: 2005888
  14. Strand specificity for UV-induced DNA repair and mutations in the Chinese hamster HPRT gene.
    Nucleic Acids Res. 1991 May 11;19(9):2411-5 PMID: 1674998
  15. DNA strand-specific mutations induced by (+/-)-3 alpha,4 beta-dihydroxy- 1 alpha,2 alpha-epoxy-1,2,3,4-tetrahydrobenzo[c]phenanthrene in the dihydrofolate reductase gene.
    Proc Natl Acad Sci U S A. 1991 Jul 1;88(13):5749-53 PMID: 2062855
  16. Xeroderma pigmentosum complementation group C cells remove pyrimidine dimers selectively from the transcribed strand of active genes.
    Mol Cell Biol. 1991 Aug;11(8):4128-34 PMID: 1649389
  17. The genetic defect in the Chinese hamster ovary cell mutant UV61 permits moderate selective repair of cyclobutane pyrimidine dimers in an expressed gene.
    Mutat Res. 1991 Sep;255(2):183-91 PMID: 1922150
  18. Xeroderma pigmentosum complementation group H falls into complementation group D.
    Mutat Res. 1991 Sep;255(2):201-8 PMID: 1922152
  19. Sensitive determination of pyrimidine dimers in DNA of UV-irradiated mammalian cells. Introduction of T4 endonuclease V into frozen and thawed cells.
    Mutat Res. 1981 Jun;82(1):173-89 PMID: 6267456
  20. Human dihydrofolate reductase gene organization. Extensive conservation of the G + C-rich 5' non-coding sequence and strong intron size divergence from homologous mammalian genes.
    J Mol Biol. 1984 Jun 25;176(2):169-87 PMID: 6235374
  21. DNA repair in an active gene: removal of pyrimidine dimers from the DHFR gene of CHO cells is much more efficient than in the genome overall.
    Cell. 1985 Feb;40(2):359-69 PMID: 3838150
  22. 5-Fluorouracil augmentation of dihydrofolate reductase gene transcripts containing intervening sequences in methotrexate-resistant KB cells.
    Mol Pharmacol. 1986 Jun;29(6):643-8 PMID: 3713705
  23. Selective removal of transcription-blocking DNA damage from the transcribed strand of the mammalian DHFR gene.
    Cell. 1987 Oct 23;51(2):241-9 PMID: 3664636
  24. Unique DNA repair properties of a xeroderma pigmentosum revertant.
    Mol Cell Biol. 1987 Sep;7(9):3353-7 PMID: 3118197
  25. Trichothiodystrophy, a human DNA repair disorder with heterogeneity in the cellular response to ultraviolet light.
    Cancer Res. 1988 Nov 1;48(21):6090-6 PMID: 2458832
  26. Strand-specific mutation spectra in repair-proficient and repair-deficient hamster cells.
    Mutat Res. 1991 Nov;251(1):143-55 PMID: 1944372
  27. Cyclobutane dimers and (6-4) photoproducts in human cells are mended with the same patch sizes.
    Photochem Photobiol. 1991 Sep;54(3):393-402 PMID: 1784640
  28. Three nonsense mutations responsible for group A xeroderma pigmentosum.
    Mutat Res. 1992 Mar;273(2):193-202 PMID: 1372102
  29. Repair of damaged DNA by extracts from a xeroderma pigmentosum complementation group A revertant and expression of a protein absent in its parental cell line.
    Nucleic Acids Res. 1992 Mar 11;20(5):991-5 PMID: 1549511
  30. (6-4) photoproducts and not cyclobutane pyrimidine dimers are the main UV-induced mutagenic lesions in Chinese hamster cells.
    Mutat Res. 1992 Jan;273(1):73-83 PMID: 1376437
  31. Isolation and characterization of cDNA clones derived from the divergently transcribed gene in the region upstream from the human dihydrofolate reductase gene.
    J Biol Chem. 1989 Jun 15;264(17):10057-64 PMID: 2722860
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.2.970-976.1993
Published
1993-02-00
Pages
970-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358981
Subset
IM
Grants
NCI NIH HHS · CA09302 · United States
NCI NIH HHS · CA44349 · United States
External Links
PubMed source DOI: 10.1128/mcb.13.2.970-976.1993 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2