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PMID: 8431959 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The smt-0 mutation which abolishes mating-type switching in fission yeast is a deletion.

Current genetics ·Vol. 23 ·No. 2 ·1993-02-00 ·Pages 184-6

Styrkársdóttir U, Egel R, Nielsen O

Abstract

Mating-type switching in the fission yeast, S. pombe, is initiated by a DNA double-strand break (DSB) between the mat1 cassette and the H1 homology box. The mat1-cis-acting mutant, smt-0, abolishes mating-type switching and is shown here to be a 263-bp deletion. This deletion starts in the middle of the H1 homology box, 31 bp from the site of the DSB, and extends into the flanking region distal to mat1. The sequence of the region distal to H1 in the wild-type is also presented. In this region we observe a bias in the distribution of purine residues between the two DNA strands.

Related Genes

mat1

MeSH Terms

Base Sequence DNA, Fungal/genetics DNA, Recombinant Genes, Fungal Genes, Mating Type, Fungal Molecular Sequence Data Mutation Schizosaccharomyces/genetics Sequence Deletion

Chemicals

DNA, Fungal DNA, Recombinant

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Styrkársdóttir U

Institute of Genetics, University of Copenhagen, Denmark.

Egel R

Nielsen O

References (15)

15 references, click to expand

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Article Info

Journal

Current genetics

Abbr.

Curr Genet

ISSN

0172-8083

DOI

10.1007/BF00352020

Published

1993-02-00

Pages

184-6

Language

English

Region

United States

NLM ID

8004904

Subset

Databases

GENBANK

S67078, X59127, X71070, X71071, X71675, X71676, X71677, X71678, X71679, X71680

External Links

PubMed source DOI: 10.1007/BF00352020 View journal details

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The smt-0 mutation which abolishes mating-type switching in fission yeast is a deletion.

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