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PMID: 8432812 Published · ppublish English Journal Article

An OspA antigen-capture enzyme-linked immunosorbent assay for detecting North American isolates of Borrelia burgdorferi in larval and nymphal Ixodes dammini.

Journal of clinical microbiology ·Vol. 31 ·No. 2 ·1993-02-00 ·Pages 272-8

Burkot TR, Wirtz RA, Luft B, Piesman J

Abstract

An antigen-capture enzyme-linked immunosorbent assay (ELISA) was developed for detecting North American isolates of Borrelia burgdorferi in larval, nymphal, and adult ticks. The assay uses an anti-OspA monoclonal antibody (H5332) for antigen capture and biotin-labelled polyclonal sera with streptavidinhorseradish peroxidase for signal generation. The assay recognized 15 of 15 North American B. burgdorferi isolates and did not cross-react with spirochete antigens of Borrelia hermsii, Borrelia turicatae, Borrelia coriaceae, or Borrelia parkeri, or with tick antigens of Ixodes dammini, Ixodes scapularis, Ixodes pacificus, Ixodes cookei, Ixodes angustus, or Ambylomma americanum. The assay, with a sensitivity of less than 150 spirochetes, can detect infections in larval, nymphal, and adult ticks. In addition to fresh ticks, B. burgdorferi infections in ticks stored frozen, dried, or in 70% ethanol can be determined with the assay.

MeSH Terms
Animals Antigens, Bacterial/analysis Antigens, Surface/analysis,immunology Bacterial Outer Membrane Proteins/analysis,immunology Bacterial Typing Techniques Bacterial Vaccines Borrelia/immunology,isolation & purification Borrelia burgdorferi Group/genetics,immunology,isolation & purification DNA, Bacterial/genetics Enzyme-Linked Immunosorbent Assay/methods,statistics & numerical data Evaluation Studies as Topic Larva/microbiology Lipoproteins North America Sensitivity and Specificity Species Specificity Ticks/microbiology
Chemicals
Antigens, Bacterial Antigens, Surface Bacterial Outer Membrane Proteins Bacterial Vaccines DNA, Bacterial Lipoproteins OspA protein
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Burkot T R
Division of Vector-Borne Infectious Diseases, Centers for Disease Control, Fort Collins, Colorado 80522.
Wirtz R A
Luft B
Piesman J
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.31.2.272-278.1993
Published
1993-02-00
Pages
272-8
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC262748
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.31.2.272-278.1993 PMC Full Text View journal details
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