Home LiteratureArticle Details
PMID: 8451170 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two non-histone proteins are associated with the promoter region and histone HI with the transcribed region of active hsp-70 genes as revealed by UV-induced DNA-protein crosslinking in vivo.

Nucleic acids research ·Vol. 21 ·No. 4 ·1993-02-25 ·Pages 1031-4

Belikov SV, Belgovsky AI, Preobrazhenskaya OV, Karpov VL, Mirzabekov AD

Abstract

We described here an approach for mapping proteins on any sequence of genomic DNA. UV-induced DNA-protein crosslinking within whole cells and the 'protein image' hybridization technique (1) were applied to test the proteins bound to different regions of the D. melanogaster hsp-70 gene. The histone H1-DNA association with the coding region is shown to be maintained, even during very intensive transcription, but is absent in the promoter. Two non-histone proteins with apparent molecular masses of 50 kD (p50) and 100 kD (p100) are crosslinked only to the active hsp-70 gene regulatory region and preferentially bind to its complementary and coding DNA strands, respectively.

Related Genes
hsp-70
MeSH Terms
Animals Binding Sites Cells, Cultured Chromosomal Proteins, Non-Histone/metabolism DNA-Binding Proteins/metabolism,radiation effects Drosophila melanogaster Heat-Shock Proteins/genetics Histones/metabolism Promoter Regions, Genetic Transcription, Genetic/physiology Ultraviolet Rays
Chemicals
Chromosomal Proteins, Non-Histone DNA-Binding Proteins Heat-Shock Proteins Histones
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Belikov S V
W.A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Belgovsky A I
Preobrazhenskaya O V
Karpov V L
Mirzabekov A D
References (24)
24 references, click to expand
  1. Two protein-binding sites in chromatin implicated in the activation of heat-shock genes.
    Nature. 1984 May 17-23;309(5965):229-34 PMID: 6325944
  2. Transcription. Riding high on the TATA box.
    Nature. 1992 Nov 5;360(6399):16-7 PMID: 1436067
  3. The transcriptional regulation of Xenopus 5s RNA genes in chromatin: the roles of active stable transcription complexes and histone H1.
    Cell. 1984 Jul;37(3):903-13 PMID: 6540147
  4. The rare transcripts of interrupted rRNA genes in Drosophila melanogaster are processed or degraded during synthesis.
    EMBO J. 1984 Jul;3(7):1541-5 PMID: 6430695
  5. Activating protein factor binds in vitro to upstream control sequences in heat shock gene chromatin.
    Nature. 1984 Sep 6-11;311(5981):81-4 PMID: 6472466
  6. High-resolution mapping of DNase I-hypersensitive sites of Drosophila heat shock genes in Drosophila melanogaster and Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Sep;4(9):1853-63 PMID: 6436689
  7. An exonuclease protection assay reveals heat-shock element and TATA box DNA-binding proteins in crude nuclear extracts.
    Nature. 1985 Sep 5-11;317(6032):84-7 PMID: 2993917
  8. The induction of gene activity in drosophilia by heat shock.
    Cell. 1979 Jun;17(2):241-54 PMID: 110462
  9. Studies of cloned sequences from four Drosophila heat shock loci.
    Cell. 1979 Dec;18(4):1359-70 PMID: 117907
  10. [Cloning and study of inserted sequences of gene 28S in Drosophila melanogaster ribosomal RNA].
    Mol Biol (Mosk). 1982 Mar-Apr;16(2):302-14 PMID: 6280035
  11. Assembly and propagation of repressed and depressed chromosomal states.
    Cell. 1985 Oct;42(3):705-11 PMID: 2996773
  12. In vivo interactions of RNA polymerase II with genes of Drosophila melanogaster.
    Mol Cell Biol. 1985 Aug;5(8):2009-18 PMID: 3018544
  13. The Saccharomyces and Drosophila heat shock transcription factors are identical in size and DNA binding properties.
    Cell. 1987 Feb 13;48(3):507-15 PMID: 3100052
  14. Purification and properties of Drosophila heat shock activator protein.
    Science. 1987 Nov 27;238(4831):1247-53 PMID: 3685975
  15. Change in the pattern of histone binding to DNA upon transcriptional activation.
    Cell. 1989 Jul 14;58(1):27-36 PMID: 2502314
  16. Mapping DNA-protein interactions by cross-linking.
    Methods Enzymol. 1989;170:386-408 PMID: 2770546
  17. Interactions of acetylated histones with DNA as revealed by UV laser induced histone-DNA crosslinking.
    Biochem Biophys Res Commun. 1989 Oct 16;164(1):304-10 PMID: 2803304
  18. Laser-induced crosslinking of histones to DNA in chromatin and core particles: implications in studying histone-DNA interactions.
    Nucleic Acids Res. 1989 Dec 11;17(23):10069-81 PMID: 2602113
  19. Histone H1 and the regulation of transcription of eukaryotic genes.
    Trends Biochem Sci. 1990 Jul;15(7):273-6 PMID: 2098005
  20. Histone H1 and transcription.
    Trends Biochem Sci. 1990 Dec;15(12):463 PMID: 2077684
  21. Distribution of high mobility group proteins 1/2, E and 14/17 and linker histones H1 and H5 on transcribed and non-transcribed regions of chicken erythrocyte chromatin.
    Nucleic Acids Res. 1991 Feb 25;19(4):717-25 PMID: 2017359
  22. Histone H1 and the conformation of transcriptionally active chromatin.
    Bioessays. 1991 Feb;13(2):87-8 PMID: 2029271
  23. What happens to nucleosomes during transcription?
    J Biol Chem. 1992 Feb 15;267(5):2837-40 PMID: 1310672
  24. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.4.1031
Published
1993-02-25
Pages
1031-4
Language
English
Region
England
NLM ID
0411011
PMCID
PMC309240
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/21.4.1031 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2