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PMID: 8464053 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Imaging frozen, hydrated acrosomal bundle from Limulus sperm at 7 A resolution with a 400 kV electron cryomicroscope.

Journal of molecular biology ·Vol. 230 ·No. 2 ·1993-03-20 ·Pages 384-6

Schmid MF, Jakana J, Matsudaira P, Chiu W

Abstract

Actin and scruin are present in a highly ordered array in the acrosomal bundle from Limulus sperm. Frozen, hydrated acrosomal bundle can be imaged in a 400 kV electron cryomicroscope using the spot-scan technique. Quantitative analysis of the h0l projection images shows diffraction data beyond 7 A.

MeSH Terms

Acrosome/ultrastructure Animals Freezing Horseshoe Crabs Male Microscopy, Electron/methods Spermatozoa/ultrastructure

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Schmid M F

Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030.

Jakana J

Matsudaira P

Chiu W

Article Info

Journal

Journal of molecular biology

Abbr.

J Mol Biol

ISSN

0022-2836

DOI

10.1006/jmbi.1993.1155

Published

1993-03-20

Pages

384-6

Language

English

Region

England

NLM ID

2985088R

Subset

Grants

NIDDK NIH HHS · DK35306 · United States

NCRR NIH HHS · RR02250 · United States

External Links

PubMed source DOI: 10.1006/jmbi.1993.1155 View journal details

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Imaging frozen, hydrated acrosomal bundle from Limulus sperm at 7 A resolution with a 400 kV electron cryomicroscope.

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