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PMID: 8466978 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detection of humoral antigen and antibody by enzyme-linked immunosorbent assay in horses with experimentally induced Ehrlichia equi infection.

Corstvet RE, Gaunt SD, Karns PA, McBride JW, Battistini RA, Mauterer LA, Austin FW

Abstract

An enzyme-linked immunosorbent assay (ELISA) was used to detect antigen in plasma and antibody in serum of 3 horses inoculated with Ehrlichia equi. Clinical signs, including rectal temperature, were correlated with the antigen and antibody detection. ELISA was very efficient in detection of serum antibody. Antigen detection using monoclonal antibodies to E. equi and ELISA should be considered as a diagnostic method.

MeSH Terms
Animals Antibodies, Monoclonal Antibodies, Viral/blood Antigens, Viral/blood Ehrlichia/immunology Ehrlichiosis/blood,diagnosis,veterinary Enzyme-Linked Immunosorbent Assay/methods Horse Diseases/blood,diagnosis,immunology Horses Immunoglobulin G/blood Immunoglobulin M/blood
Chemicals
Antibodies, Monoclonal Antibodies, Viral Antigens, Viral Immunoglobulin G Immunoglobulin M
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Corstvet R E
Department of Veterinary Science, LSU Agricultural Center, Baton Rouge.
Gaunt S D
Karns P A
McBride J W
Battistini R A
Mauterer L A
Austin F W
Article Info
Journal
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Abbr.
J Vet Diagn Invest
ISSN
1040-6387
Published
1993-01-00
Pages
37-9
Language
English
Region
United States
NLM ID
9011490
Subset
IM
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