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PMID: 8467810 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Depletion of casein kinase II by antisense oligonucleotide prevents neuritogenesis in neuroblastoma cells.

The EMBO journal ·Vol. 12 ·No. 4 ·1993-04-00 ·Pages 1633-40

Ulloa L, Díaz-Nido J, Avila J

Abstract

Casein kinase II is a multifunctional protein kinase which has been implicated in the regulation of cell growth and differentiation. This enzyme is much more abundant in neurons than in any other cell type. The treatment of neuroblastoma cells with an antisense oligodeoxyribonucleotide which specifically results in the depletion of casein kinase II catalytic subunits blocks neuritogenesis. Accordingly, this enzyme may perform an essential role during neurite growth in developing neurons. Casein kinase II depletion induced by antisense oligodeoxyribonucleotide is accompanied by a site-specific dephosphorylation of microtubule-associated protein MAP1B (also referred to as MAP5, MAP1.X or MAP1.2), which is paralleled by a release of MAP1B from microtubules. We therefore propose that phosphorylation by casein kinase II may be required for the proper MAP1B functioning in the promotion of the assembly of microtubules which constitute the cytoskeletal scaffolding of growing axon-like neurites.

Related Genes
CKII MAP1B
MeSH Terms
Animals Base Sequence Casein Kinase II Cell Differentiation Fluorescent Antibody Technique In Vitro Techniques Mice Microtubule-Associated Proteins/metabolism Microtubules/metabolism Molecular Sequence Data Neurons/cytology Oligodeoxyribonucleotides/chemistry Oligonucleotides, Antisense Phosphorylation Protein Serine-Threonine Kinases/physiology Time Factors Tumor Cells, Cultured
Chemicals
Microtubule-Associated Proteins Oligodeoxyribonucleotides Oligonucleotides, Antisense microtubule-associated protein 1B Casein Kinase II Protein Serine-Threonine Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ulloa L
Centro de Biología Molecular (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma, Madrid, Spain.
Díaz-Nido J
Avila J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-04-00
Pages
1633-40
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413377
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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