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PMID: 8468301 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Sequence of the gene coding for ammonia monooxygenase in Nitrosomonas europaea.

Journal of bacteriology ·Vol. 175 ·No. 8 ·1993-04-00 ·Pages 2436-44

McTavish H, Fuchs JA, Hooper AB

Abstract

Nitrosomonas europaea, a chemolithotrophic bacterium, was found to contain two copies of the gene coding for the presumed active site polypeptide of ammonia monooxygenase, the 32-kDa acetylene-binding polypeptide. One copy of this gene was cloned, and its complete nucleotide sequence is presented. Immediately downstream of this gene, in the same operon, is the gene for a 40-kDa polypeptide that copurifies with the ammonia monooxygenase acetylene-binding polypeptide. The sequence of the first 692 nucleotides of this structural gene, coding for about two-thirds of the protein, is presented. These sequences are the first sequences of protein-encoding genes from an ammonia-oxidizing autotrophic nitrifying bacterium. The two protein sequences are not homologous with the sequences of any other monooxygenase. From radioactive labelling of ammonia monooxygenase with [14C]acetylene it was determined that there are 23 nmol of ammonia monooxygenase per g of cells. The kcat of ammonia monooxygenase for NH3 in vivo was calculated to be 20 s-1.

Related Genes
amoA amoB
MeSH Terms
Acetylene/metabolism Amino Acid Sequence Amino Acids/analysis Base Sequence Cloning, Molecular Genes, Bacterial Molecular Sequence Data Nitrosomonas/enzymology Operon Oxidoreductases/chemistry,genetics,isolation & purification
Chemicals
Amino Acids Oxidoreductases ammonia monooxygenase Acetylene
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McTavish H
Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108.
Fuchs J A
Hooper A B
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34 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.8.2436-2444.1993
Published
1993-04-00
Pages
2436-44
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC204533
Subset
IM
Databases
GENBANK
L08050
External Links
PubMed source DOI: 10.1128/jb.175.8.2436-2444.1993 PMC Full Text View journal details
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