The Ada protein is a class I transcription factor of Escherichia coli.
The methylated Ada protein of Escherichia coli, a regulatory protein for the adaptive response, binds to a target DNA from positions -62 to -31 upstream of the ada gene and facilitates the binding of RNA polymerase to the promoter. Mutant RNA polymerases consisting of C-terminal-deleted alpha subunits are virtually inactive in response to activation by the Ada protein. Thus, we conclude that the Ada protein is a class I transcription factor which requires the C-terminal region of the RNA polymerase alpha subunit for transcription activation.
14 references, click to expand
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Purification and structure of the intact Ada regulatory protein of Escherichia coli K12, O6-methylguanine-DNA methyltransferase.J Biol Chem. 1985 Jun 25;260(12):7281-8 PMID: 2987251
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Cloning of the E. coli O6-methylguanine and methylphosphotriester methyltransferase gene using a functional DNA repair assay.Nucleic Acids Res. 1985 Mar 25;13(6):1939-52 PMID: 3889845
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Inducible DNA repair systems.Annu Rev Biochem. 1985;54:425-57 PMID: 3896123
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Functional sites of the Ada regulatory protein of Escherichia coli. Analysis by amino acid substitutions.J Mol Biol. 1988 May 20;201(2):261-71 PMID: 3047400
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Expression of the ada gene of Escherichia coli in response to alkylating agents. Identification of transcriptional regulatory elements.J Mol Biol. 1988 Aug 5;202(3):483-94 PMID: 3139888
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Role of the sigma 70 subunit of RNA polymerase in transcriptional activation by activator protein PhoB in Escherichia coli.Genes Dev. 1993 Jan;7(1):149-60 PMID: 8422984
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Positive and negative regulation of transcription by a cleavage product of Ada protein.J Mol Biol. 1990 Nov 20;216(2):261-73 PMID: 2254928
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Bipartite functional map of the E. coli RNA polymerase alpha subunit: involvement of the C-terminal region in transcription activation by cAMP-CRP.Cell. 1991 Jun 14;65(6):1015-22 PMID: 1646077
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Functional map of the alpha subunit of Escherichia coli RNA polymerase: two modes of transcription activation by positive factors.Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):8958-62 PMID: 1833768
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Activation defects caused by mutations in Escherichia coli rpoA are promoter specific.J Bacteriol. 1992 Aug;174(15):5156-60 PMID: 1629175
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Mapping the cAMP receptor protein contact site on the alpha subunit of Escherichia coli RNA polymerase.Mol Microbiol. 1992 Sep;6(18):2599-605 PMID: 1333035
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Role of the RNA polymerase alpha subunit in transcription activation.Mol Microbiol. 1992 Nov;6(22):3283-8 PMID: 1484484
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Regulation of expression of the ada gene controlling the adaptive response. Interactions with the ada promoter of the Ada protein and RNA polymerase.J Mol Biol. 1989 Jan 20;205(2):373-85 PMID: 2648001
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