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PMID: 8478116 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation, expression, and nucleotide sequencing of the pilin structural gene of the Brazilian purpuric fever clone of Haemophilus influenzae biogroup aegyptius.

Infection and immunity ·Vol. 61 ·No. 5 ·1993-05-00 ·Pages 2233-7

St Geme JW, Falkow S

Abstract

In this study we isolated the pilin gene from the Brazilian purpuric fever (BPF) clone of Haemophilus influenzae biogroup aegyptius, expressed the gene in Escherichia coli, and determined its nucleotide sequence. Comparison of the nucleotide sequence of the BPF pilin gene with the sequences of pilin genes from strains of H. influenzae sensu stricto demonstrated a high degree of identity. Consistent with this observation, hemagglutination inhibition studies performed with a series of glycoconjugates indicated that BPF pili and H. influenzae type b pili possess the same erythrocyte receptor specificity.

MeSH Terms

Amino Acid Sequence Bacterial Outer Membrane Proteins/genetics Base Sequence Blotting, Southern Brazil Cloning, Molecular Fimbriae Proteins Genes, Bacterial Glycoconjugates/pharmacology Haemophilus influenzae/genetics Hemagglutination/drug effects Humans Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Purpura/microbiology Sequence Alignment Sequence Homology, Amino Acid

Chemicals

Bacterial Outer Membrane Proteins Glycoconjugates Oligodeoxyribonucleotides Fimbriae Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

St Geme J W

Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri.

Falkow S

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22 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.61.5.2233-2237.1993

Published

1993-05-00

Pages

2233-7

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC280830

Subset

Grants

NIAID NIH HHS · AI26195 · United States

NICHD NIH HHS · HD29678 · United States

External Links

PubMed source DOI: 10.1128/iai.61.5.2233-2237.1993 PMC Full Text View journal details

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Isolation, expression, and nucleotide sequencing of the pilin structural gene of the Brazilian purpuric fever clone of Haemophilus influenzae biogroup aegyptius.

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