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PMID: 8483450 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A new yeast gene with a myosin-like heptad repeat structure.

Molecular & general genetics : MGG ·Vol. 237 ·No. 3 ·1993-03-00 ·Pages 359-69

Kölling R, Nguyen T, Chen EY, Botstein D

Abstract

We isolated a gene encoding a 218 kDa myosin-like protein from Saccharomyces cerevisiae using a monoclonal antibody directed against human platelet myosin as a probe. The protein sequence encoded by the MLP1 gene (for myosin-like protein) contains extensive stretches of a heptad-repeat pattern suggesting that the protein can form coiled coils typical of myosins. Immunolocalization experiments using affinity-purified antibodies raised against a TrpE-MLP1 fusion protein showed a dot-like structure adjacent to the nucleus in yeast cells bearing the MLP1 gene on a multicopy plasmid. In mouse epithelial cells the yeast anti-MLP1 antibodies stained the nucleus. Mutants bearing disruptions of the MLP1 gene were viable, but more sensitive to ultraviolet light than wild-type strains, suggesting an involvement of MLP1 in DNA repair. The MLP1 gene was mapped to chromosome 11, 25 cM from met1.

Related Genes
MLP1
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Cloning, Molecular DNA, Fungal Fluorescent Antibody Technique Fungal Proteins/genetics Genes, Fungal Humans Molecular Sequence Data Myosins/genetics Nuclear Proteins RNA-Binding Proteins Repetitive Sequences, Nucleic Acid Restriction Mapping Saccharomyces cerevisiae/genetics,radiation effects Saccharomyces cerevisiae Proteins Ultraviolet Rays
Chemicals
DNA, Fungal Fungal Proteins MLP1 protein, S cerevisiae Nuclear Proteins RNA-Binding Proteins Saccharomyces cerevisiae Proteins Myosins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kölling R
Department of Cell Genetics, Genentech Inc., South San Francisco, CA 94305.
Nguyen T
Chen E Y
Botstein D
References (28)
28 references, click to expand
  1. Two functional alpha-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins.
    Mol Cell Biol. 1986 Nov;6(11):3711-21 PMID: 3025610
  2. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector.
    Gene. 1987;60(2-3):237-43 PMID: 3327750
  3. Molecular cloning of the actin gene from yeast Saccharomyces cerevisiae.
    Nucleic Acids Res. 1980 Mar 11;8(5):1043-59 PMID: 7003553
  4. Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3912-6 PMID: 7001447
  5. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  6. tpr homologues activate met and raf.
    Oncogene. 1988 Jun;2(6):617-9 PMID: 3387099
  7. Disruption of the single tropomyosin gene in yeast results in the disappearance of actin cables from the cytoskeleton.
    Cell. 1989 Apr 21;57(2):233-42 PMID: 2649250
  8. Construction of high copy yeast vectors using 2-microns circle sequences.
    Methods Enzymol. 1983;101:307-25 PMID: 6310334
  9. The intron-containing gene for yeast profilin (PFY) encodes a vital function.
    Mol Cell Biol. 1988 Dec;8(12):5108-15 PMID: 3072473
  10. Isolation of the beta-tubulin gene from yeast and demonstration of its essential function in vivo.
    Cell. 1983 May;33(1):211-9 PMID: 6380751
  11. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  12. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  13. Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis.
    Science. 1986 Oct 17;234(4774):364-8 PMID: 2876518
  14. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  15. Yeast actin-binding proteins: evidence for a role in morphogenesis.
    J Cell Biol. 1988 Dec;107(6 Pt 2):2551-61 PMID: 3060468
  16. Assembly of the mitochondrial membrane system. CBP6, a yeast nuclear gene necessary for synthesis of cytochrome b.
    J Biol Chem. 1985 Feb 10;260(3):1513-20 PMID: 2981859
  17. Rat c-raf oncogene activation by a rearrangement that produces a fused protein.
    Mol Cell Biol. 1987 Mar;7(3):1226-32 PMID: 3550433
  18. Yeast RNA polymerase II genes: isolation with antibody probes.
    Science. 1983 Nov 18;222(4625):778-82 PMID: 6356359
  19. Disruption of the actin cytoskeleton in yeast capping protein mutants.
    Nature. 1990 Mar 22;344(6264):352-4 PMID: 2179733
  20. Prediction of protein conformation.
    Biochemistry. 1974 Jan 15;13(2):222-45 PMID: 4358940
  21. The yeast RAD50 gene encodes a predicted 153-kD protein containing a purine nucleotide-binding domain and two large heptad-repeat regions.
    Genetics. 1989 May;122(1):47-57 PMID: 2659437
  22. The yeast MYO1 gene encoding a myosin-like protein required for cell division.
    EMBO J. 1987 Nov;6(11):3499-505 PMID: 3322809
  23. Cloned viral protein vaccine for foot-and-mouth disease: responses in cattle and swine.
    Science. 1981 Dec 4;214(4525):1125-9 PMID: 6272395
  24. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
    Anal Biochem. 1981 Apr;112(2):195-203 PMID: 6266278
  25. Identification of three coated vesicle components as alpha- and beta-tubulin linked to a phosphorylated 50,000-dalton polypeptide.
    J Cell Biol. 1983 Jul;97(1):40-7 PMID: 6134738
  26. Fluorescence microscopy methods for yeast.
    Methods Cell Biol. 1989;31:357-435 PMID: 2476649
  27. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  28. Identification of myosin heavy chain in Saccharomyces cerevisiae.
    Nature. 1985 Jul 4-10;316(6023):83-5 PMID: 3892306
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00279439
Published
1993-03-00
Pages
359-69
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
L01992
External Links
PubMed source DOI: 10.1007/BF00279439
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