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PMID: 8483934 Published · ppublish English Comparative Study Journal Article

Cloning and characterization of a fourth human somatostatin receptor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 9 ·1993-05-01 ·Pages 4196-200

Rohrer L, Raulf F, Bruns C, Buettner R, Hofstaedter F, Schüle R

Abstract

We have isolated a gene coding for a fourth human somatostatin (somatotropin release-inhibiting factor) receptor. This additional somatostatin receptor (hSSTR4) is specifically expressed in human fetal and adult brain and lung tissue. The deduced amino acid sequence of the receptor displays both sequence and structural homology to three cloned somatostatin receptors as well as to other members of the family of GTP-binding-protein-coupled seven-helix transmembrane-spanning receptors. Pharmacological characterization of the expressed receptor reveals specific, high-affinity binding of somatostatin 14 and somatostatin 28. Surprisingly, several well-characterized synthetic somatostatin analogs fail to exhibit high-affinity binding to hSSTR4, indicating the existence of pharmacologically different receptor subtypes. Our data suggest that the diverse biological effects exerted by somatostatin are mediated by a family of receptors with discrete patterns of expression and different pharmacological properties.

Related Genes
SSTR4 hSSTR4
MeSH Terms
Amino Acid Sequence Base Sequence Binding, Competitive Blotting, Northern Brain/metabolism Cloning, Molecular/methods Genomic Library Humans Kinetics Lung/metabolism Molecular Sequence Data Oligodeoxyribonucleotides Organ Specificity Polymerase Chain Reaction/methods Protein Conformation Receptors, Somatostatin/genetics,isolation & purification,metabolism Recombinant Proteins/metabolism Restriction Mapping Sequence Homology, Amino Acid Software
Chemicals
Oligodeoxyribonucleotides Receptors, Somatostatin Recombinant Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rohrer L
Preclinical Research, Sandoz Pharma, Ltd., Basel, Switzerland.
Raulf F
Bruns C
Buettner R
Hofstaedter F
Schüle R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.9.4196
Published
1993-05-01
Pages
4196-200
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46473
Subset
IM
Databases
GENBANK
L07833
External Links
PubMed source DOI: 10.1073/pnas.90.9.4196 PMC Full Text View journal details
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