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PMID: 8484739 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The sub-cellular localization of annexin V in cultured chick-embryo fibroblasts.

The Biochemical journal ·Vol. 291 ( Pt 2) ·1993-04-15 ·Pages 595-600

Koster JJ, Boustead CM, Middleton CA, Walker JH

Abstract

The Ca(2+)- and phospholipid-binding protein, annexin V, has been shown by an immune assay to represent 0.4% of total cell protein in cultured chick-embryo fibroblasts. Immunofluorescent localization studies indicate that in primary cultures the protein is abundant in the cytoplasm of the cells and also extends into the nucleus. Nuclear staining is no longer detectable, however, in approx. 25% of the cells following sub-culture. Sub-populations of annexin V are associated with cytoskeletal structures and with the inner face of the plasma membrane in a Ca(2+)-independent manner. In addition, we report results indicating the secretion of annexin V from this cell type.

MeSH Terms
Animals Annexin A5/analysis,metabolism Calcium/pharmacology Cell Membrane/chemistry Cell Nucleus/chemistry Cells, Cultured Chick Embryo Cytoskeleton/chemistry Fibroblasts/chemistry,ultrastructure Fluorescent Antibody Technique Immunoblotting
Chemicals
Annexin A5 Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Koster J J
Department of Biochemistry and Molecular Biology, University of Leeds, U.K.
Boustead C M
Middleton C A
Walker J H
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2910595
Published
1993-04-15
Pages
595-600
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1132565
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1042/bj2910595 PMC Full Text View journal details
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