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PMID: 8500886 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The putative arthritogenic cationic 19-kilodalton antigen of Yersinia enterocolitica is a urease beta-subunit.

Infection and immunity ·Vol. 61 ·No. 6 ·1993-06-00 ·Pages 2498-504

Skurnik M, Batsford S, Mertz A, Schiltz E, Toivanen P

Abstract

The gene coding for a putative 19-kDa arthritogenic antigen of Yersinia enterocolitica O:3 (A. K. H. Mertz et al., J. Clin. Invest. 87:632-642, 1991) was cloned and sequenced after amplification of part of the gene by the polymerase chain reaction using degenerate primers, inferred from the amino acid sequence. The deduced amino acid sequence of the antigen showed similarity to small subunits of ureases from several different organisms, including the jack bean urease. Screening of a genomic library of Y. enterocolitica O:3 with a 19-kDa-antigen-specific DNA probe allowed recombinant clones containing the entire urease operon to be obtained. These clones expressed urease activity in Escherichia coli.

Related Genes
yeuA yeuB yeuC
MeSH Terms
Amino Acid Sequence Antigens, Bacterial/chemistry,genetics,immunology Arthritis, Infectious/microbiology Bacterial Proteins/genetics,immunology,metabolism Base Sequence Cloning, Molecular DNA, Bacterial Genes, Bacterial Humans Molecular Sequence Data Polymerase Chain Reaction Sequence Alignment Urease/chemistry,genetics,immunology,metabolism Yersinia Infections/microbiology Yersinia enterocolitica/enzymology,genetics,immunology
Chemicals
Antigens, Bacterial Bacterial Proteins DNA, Bacterial UREB protein, Yersinia enterocolitica Urease yeuA protein, Yersinia enterocolitica yeuC protein, Yersinia enterocolitica
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Skurnik M
Department of Medical Microbiology, Turku University, Finland.
Batsford S
Mertz A
Schiltz E
Toivanen P
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26 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.61.6.2498-2504.1993
Published
1993-06-00
Pages
2498-504
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC280875
Subset
IM
Databases
GENBANK
Z18865
External Links
PubMed source DOI: 10.1128/iai.61.6.2498-2504.1993 PMC Full Text View journal details
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