An efficient 1-tube PCR method for internal site-directed mutagenesis of large amplified molecules.

Nucleic acids research ·Vol. 21 ·No. 9 ·1993-05-11 ·Pages 2277-8

Marini F, Naeem A, Lapeyre JN

Abstract

暂无摘要

MeSH Terms

Base Sequence DNA, Single-Stranded Molecular Sequence Data Mutagenesis, Site-Directed Polymerase Chain Reaction/methods

Chemicals

DNA, Single-Stranded

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Marini F

Department of Molecular Pathology, University of Texas M.D. Anderson Cancer Center, Houston 77030.

Naeem A

Lapeyre J N

References (7)

7 references, click to expand

Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.
Nucleic Acids Res. 1988 Oct 25;16(20):9677-86 PMID: 2460825
Rapid insertional mutagenesis of DNA by polymerase chain reaction (PCR).
Nucleic Acids Res. 1989 Jul 11;17(13):5404 PMID: 2548160
A general and rapid mutagenesis method using polymerase chain reaction.
Gene. 1990 Jul 2;91(1):143-7 PMID: 2169445
Double-stranded DNA segments can efficiently prime the amplification of human genomic DNA.
Nucleic Acids Res. 1992 Sep 25;20(18):4937-8 PMID: 1408814
A general method for rapid site-directed mutagenesis using the polymerase chain reaction.
Gene. 1990 Nov 30;96(1):125-8 PMID: 2265750
Effects of different DNA polymerases in ligation-mediated PCR: enhanced genomic sequencing and in vivo footprinting.
Proc Natl Acad Sci U S A. 1992 Feb 1;89(3):1021-5 PMID: 1736283
Site-specific mutagenesis using asymmetric polymerase chain reaction and a single mutant primer.
Nucleic Acids Res. 1990 Dec 25;18(24):7433-8 PMID: 2259632

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

Published

1993-05-11

Pages

2277-8

Language

English

Region

England

NLM ID

0411011

PMCID

PMC309512

Subset

External Links

Analysis Services