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PMID: 8506309 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Genes on chromosomes 4, 9, and 19 involved in 11q23 abnormalities in acute leukemia share sequence homology and/or common motifs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 10 ·1993-05-15 ·Pages 4631-5

Nakamura T, Alder H, Gu Y, Prasad R, Canaani O, Kamada N, Gale RP, Lange B, Crist WM, Nowell PC

Abstract

Chromosome translocations involving band 11q23 are associated with human acute leukemias. These translocations fuse the ALL-1 gene, homolog of Drosophila trithorax and located at chromosome band 11q23, to genes from a variety of chromosomes. We cloned and sequenced cDNAs derived from transcripts of the AF-4 and AF-9 genes involved in the most common chromosome abnormalities, t(4:11)(q21:q23) and t(9:11)(p22:q23), respectively. Sequence analysis indicates high homology between the AF-9 gene protein product and the protein encoded by the ENL gene fused to ALL-1 in (11:19) chromosome translocations. AF-4, AF-9, and ENL proteins contain nuclear targeting sequences as well as serine-rich and proline-rich regions. Stretches abundant in basic amino acids are also present in the three proteins. These results suggest that the different proteins fused to ALL-1 polypeptide(s) provide similar functional domains.

MeSH Terms
Acute Disease Amino Acid Sequence Base Sequence Chromosome Aberrations/genetics Chromosome Disorders Chromosomes, Human, Pair 11 Chromosomes, Human, Pair 19 Chromosomes, Human, Pair 4 Chromosomes, Human, Pair 9 Cloning, Molecular DNA/genetics DNA, Neoplasm/genetics DNA-Binding Proteins/genetics Genes Humans Leukemia/genetics Molecular Sequence Data Neoplasm Proteins Nuclear Proteins/genetics Oligodeoxyribonucleotides/chemistry Recombinant Fusion Proteins/genetics Restriction Mapping Sequence Alignment Sequence Homology, Amino Acid Transcription Factors Transcriptional Elongation Factors Translocation, Genetic
Chemicals
DNA, Neoplasm DNA-Binding Proteins MLLT1 protein, human MLLT3 protein, human Neoplasm Proteins Nuclear Proteins Oligodeoxyribonucleotides Recombinant Fusion Proteins Transcription Factors Transcriptional Elongation Factors AFF1 protein, human DNA
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Nakamura T
Jefferson Cancer Institute, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107.
Alder H
Gu Y
Prasad R
Canaani O
Kamada N
Gale R P
Lange B
Crist W M
Nowell P C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.10.4631
Published
1993-05-15
Pages
4631-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46566
Subset
IM
Grants
NCI NIH HHS · CA39860 · United States
Databases
GENBANK
L13743, L13744, L13773
External Links
PubMed source DOI: 10.1073/pnas.90.10.4631 PMC Full Text View journal details
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