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PMID: 8506348 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Modulation of the interaction between G-actin and thymosin beta 4 by the ATP/ADP ratio: possible implication in the regulation of actin dynamics.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 11 ·1993-06-01 ·Pages 5034-8

Carlier MF, Jean C, Rieger KJ, Lenfant M, Pantaloni D

Abstract

The interaction of G-actin with thymosin beta 4 (T beta 4), the major G-actin-sequestering protein in motile and proliferating cells, has been analyzed in vitro. T beta 4 is found to have a 50-fold higher affinity for MgATP-actin than for MgADP-actin. These results imply that in resting platelets and neutrophils, actin is sequestered by T beta 4 as MgATP-G-actin. Kinetic experiments and theoretical calculations demonstrate that this ATP/ADP dependence of T beta 4 affinity for G-actin can generate a mechanism of desequestration of G-actin by ADP, in the presence of physiological concentrations of T beta 4 (approximately 0.1 mM). The desequestration of G-actin by ADP is kinetically enhanced by profilin, which accelerates the dissociation of ATP from G-actin. Whether a local drop in the ATP/ADP ratio can allow local, transient desequestration and polymerization of actin either close to the plasma membrane, following platelet or neutrophil stimulation, or behind the Listeria bacterium in the host cell, while the surrounding cytoplasm contains sequestered ATP-G-actin, is an open issue raised by the present work.

MeSH Terms
Actins/metabolism Adenosine Diphosphate/metabolism Adenosine Triphosphate/metabolism Animals Cell Division Contractile Proteins/pharmacology Kinetics Mathematics Microfilament Proteins/isolation & purification,pharmacology Models, Biological Muscles/metabolism Profilins Sheep Spleen/metabolism Thymosin/metabolism
Chemicals
Actins Contractile Proteins Microfilament Proteins Profilins thymosin beta(4) Thymosin Adenosine Diphosphate Adenosine Triphosphate
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Carlier M F
Laboratoire d'Enzymologie, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
Jean C
Rieger K J
Lenfant M
Pantaloni D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.11.5034
Published
1993-06-01
Pages
5034-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46648
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.90.11.5034 PMC Full Text View journal details
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