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PMID: 8506376 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Myocyte enhancer factor (MEF) 2C: a tissue-restricted member of the MEF-2 family of transcription factors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 11 ·1993-06-01 ·Pages 5282-6

Martin JF, Schwarz JJ, Olson EN

Abstract

MEF-2 is a muscle-specific DNA binding activity that recognizes an A+T-rich sequence found in the control regions of numerous muscle-specific genes. The recent cloning of MEF-2 showed that it belongs to the MADS (MCM1, Agamous, Deficiens, and serum-response factor) box family of transcription factors and that MEF-2 mRNA is expressed ubiquitously. Here we describe the cloning of a member of the MEF-2 gene family, referred to as MEF-2C, that is nearly identical to other MEF-2 gene products in the MADS box but diverges from other members of the family outside of this domain. MEF-2C binds the MEF-2 site with high affinity and can activate transcription of a reporter gene linked to tandem copies of that site. In contrast to previously described members of the MEF-2 family, MEF-2C transcripts are highly enriched in skeletal muscle, spleen, and brain of adult mice and are upregulated during myoblast differentiation. These results suggest that the MEF-2 site is a target for a diverse family of proteins that regulates transcription in a variety of cell types.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Brain/metabolism Cell Line Cloning, Molecular Creatine Kinase/metabolism DNA/genetics,isolation & purification DNA-Binding Proteins/genetics,metabolism Exons Humans MADS Domain Proteins MEF2 Transcription Factors Mice Molecular Sequence Data Multigene Family Muscles/metabolism Myocardium/metabolism Myogenic Regulatory Factors Oligodeoxyribonucleotides Organ Specificity Polymerase Chain Reaction Protein Biosynthesis RNA, Messenger/metabolism Restriction Mapping Sequence Homology, Amino Acid Spleen/metabolism Transcription Factors/genetics,metabolism Transcription, Genetic Transfection
Chemicals
DNA-Binding Proteins MADS Domain Proteins MEF2 Transcription Factors MEF2C protein, human Mef2c protein, mouse Myogenic Regulatory Factors Oligodeoxyribonucleotides RNA, Messenger Transcription Factors DNA Creatine Kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Martin J F
Department of Biochemistry and Molecular Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Schwarz J J
Olson E N
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.11.5282
Published
1993-06-01
Pages
5282-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46700
Subset
IM
Grants
NIAMS NIH HHS · AR39849 · United States
NIAMS NIH HHS · AR40339 · United States
NCI NIH HHS · CA-16672 · United States
External Links
PubMed source DOI: 10.1073/pnas.90.11.5282 PMC Full Text View journal details
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