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PMID: 8514410 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence encoding the mannose-fucose-resistant hemagglutinin of Vibrio cholerae O1 and construction of a mutant.

Infection and immunity ·Vol. 61 ·No. 7 ·1993-07-00 ·Pages 3032-7

Franzon VL, Barker A, Manning PA

Abstract

The region of DNA encoding the mannose-fucose-resistant hemagglutinin (MFRHA) of Vibrio cholerae O1 has been localized, and the nucleotide sequence has been determined. The region contains a single open reading frame encoding 230 amino acids, corresponding to a protein of 26.9 kDa. The N terminus of this protein is atypical for a protein localized in the outer membrane. A mutant lacking MFRHA activity has been constructed by allelic exchange after inactivation via the insertion of a kanamycin resistance gene cartridge. The MFRHA-negative mutant has been assessed for virulence in the infant mouse cholera model. This mutant shows a marked defect in its ability to persist in the infant mouse gut and is incapable of competing with the wild-type organism, even when given in 25-fold excess. This defect also leads to a > 100-fold increase in the 50% lethal dose. These data suggest that the MFRHA is an important colonization factor in the infant mouse model.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Fucose/pharmacology Hemagglutinins/chemistry,genetics,toxicity Lectins Mannose/pharmacology Mice Molecular Sequence Data Mutation Vibrio cholerae/genetics,pathogenicity Virulence
Chemicals
Hemagglutinins Lectins mannose-fucose-resistant hemagglutinin Fucose Mannose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Franzon V L
Department of Microbiology and Immunology, University of Adelaide, Australia.
Barker A
Manning P A
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.61.7.3032-3037.1993
Published
1993-07-00
Pages
3032-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC280956
Subset
IM
Databases
GENBANK
X64097
External Links
PubMed source DOI: 10.1128/iai.61.7.3032-3037.1993 PMC Full Text View journal details
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