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PMID: 8517713 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mobilization of the penicillinase gene in Enterococcus faecalis.

Antimicrobial agents and chemotherapy ·Vol. 37 ·No. 5 ·1993-05-00 ·Pages 1187-9

Chow JW, Perri MB, Thal LA, Zervos MJ

Abstract

Enterococcus faecalis SF4855 is a beta-lactamase-producing isolate resistant to high levels of gentamicin, with determinants for these resistances on the chromosome. SF4855 transferred both determinants into E. faecalis FA2-2 and UV202 at a frequency of 10(-9) in the presence of the MLS plasmid pYN120. beta-Lactamase and gentamicin resistance probes hybridized to three locations on the chromosome of FA2-2 transconjugants on contour-clamped homogeneous electric field electrophoresis. The study results suggest mobilization of the beta-lactamase determinant.

MeSH Terms
Chromosomes, Bacterial DNA, Bacterial/analysis Enterococcus faecalis/drug effects,enzymology,genetics Genes, Bacterial Gentamicins/pharmacology Microbial Sensitivity Tests Penicillinase/genetics Plasmids
Chemicals
DNA, Bacterial Gentamicins Penicillinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chow J W
Department of Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201.
Perri M B
Thal L A
Zervos M J
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.37.5.1187
Published
1993-05-00
Pages
1187-9
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC187930
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.37.5.1187 PMC Full Text View journal details
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