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PMID: 8534090 Published · ppublish English Journal Article

Cloning and expression of a novel toxin gene from Bacillus thuringiensis subsp. jegathesan encoding a highly mosquitocidal protein.

Applied and environmental microbiology ·Vol. 61 ·No. 12 ·1995-12-00 ·Pages 4230-5

Delécluse A, Rosso ML, Ragni A

Abstract

A gene, designated cry11B, encoding a 81,293-Da crystal protein of Bacillus thuringiensis subsp. jegathesan was cloned by using a gene-specific oligonucleotide probe. The sequence of the Cry11B protein, as deduced from the sequence of the cry11B gene, contains large regions of similarity with the Cry11A toxin (previously CryIVD) from B. thuringiensis subsp. israelensis. The Cry11B protein was immunologically related to both Cry11A and Cry4A proteins. The cry11B gene was expressed in a nontoxic strain of B. thuringiensis, in which Cry11B was produced in large amounts during sporulation and accumulated as inclusions. Purified Cry11B inclusions were highly toxic for mosquito larvae of the species Aedes aegypti, Culex pipiens, and Anopheles stephensi. The activity of Cry11B toxin was higher than that of Cry11A and similar to that of the native crystals from B. thuringiensis subsp. jegathesan, which contain at least seven polypeptides.

MeSH Terms
Amino Acid Sequence Animals Bacillus thuringiensis/genetics,metabolism Bacterial Toxins/genetics Base Sequence Cloning, Molecular Culicidae/microbiology Genes, Bacterial Molecular Sequence Data Sequence Alignment
Chemicals
Bacterial Toxins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Delécluse A
Unité des Bactéries Entomopathogènes, Institut Pasteur, Paris, France.
Rosso M L
Ragni A
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.61.12.4230-4235.1995
Published
1995-12-00
Pages
4230-5
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC167734
Subset
IM
Databases
GENBANK
X86902
External Links
PubMed source DOI: 10.1128/aem.61.12.4230-4235.1995 PMC Full Text View journal details
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