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PMID: 8536311 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Schizosaccharomyces pombe pac2+ controls the onset of sexual development via a pathway independent of the cAMP cascade.

Current genetics ·Vol. 28 ·No. 1 ·1995-06-00 ·Pages 32-8

Kunitomo H, Sugimoto A, Wilkinson CR, Yamamoto M

Abstract

The Schizosaccharomyces pombe pac2 gene encodes a protein of 235 amino acids not similar to any protein of known function. Cells over-expressing pac2 were poor in mating and sporulation. Expression of ste11, which encodes a key transcription factor for sexual development, was not inducible by nitrogen starvation in these cells. Cells defective in pac2 could express ste11 and enter sexual development under incomplete starvation conditions. Although expression of ste11 is regulated primarily by the cAMP cascade, genetic analysis indicated that this cascade and pac2 can partially compensate for each other in the regulation of sexual development, and that neither of them is epistatic over the other. Thus, Pac2 appears to control ste11 expression via a signaling pathway independent of the cAMP cascade.

MeSH Terms
Amino Acid Sequence Base Sequence Cyclic AMP/metabolism DNA, Complementary DNA, Fungal Fungal Proteins/genetics,physiology Molecular Sequence Data Phenotype Schizosaccharomyces/genetics,physiology Schizosaccharomyces pombe Proteins Transcription Factors/genetics
Chemicals
DNA, Complementary DNA, Fungal Fungal Proteins PAC2 protein, S pombe Schizosaccharomyces pombe Proteins Transcription Factors ste11 protein, S pombe Cyclic AMP
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kunitomo H
Department of Biophysics and Biochemistry, School of Science, University of Tokyo, Japan.
Sugimoto A
Wilkinson C R
Yamamoto M
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27 references, click to expand
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00311879
Published
1995-06-00
Pages
32-8
Language
English
Region
United States
NLM ID
8004904
Subset
IM
Databases
GENBANK
D43748
External Links
PubMed source DOI: 10.1007/BF00311879 View journal details
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