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PMID: 8536800 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Review

PSF and CMF, autocrine factors that regulate gene expression during growth and early development of Dictyostelium.

Experientia ·Vol. 51 ·No. 12 ·1995-12-18 ·Pages 1124-34

Clarke M, Gomer RH

Abstract

Throughout growth and development, Dictyostelium cells secrete autocrine factors that accumulate in proportion to cell density. At sufficient concentration, these factors cause changes in gene expression. Vegetative Dictyostelium cells continuously secrete prestarvation factor (PSF). The bacteria upon which the cells feed inhibit their response to PSF, allowing the cells to monitor their own density in relation to that of their food supply. At high PSF/bacteria ratios, which occur during late exponential growth, PSF induces the expression of several genes whose products are needed for cell aggregation. When the food supply has been depleted, PSF production declines, and a second density-sensing pathway is activated. Starving cells secrete conditioned medium factor (CMF), a glycoprotein of Mr 80 kDa that is essential for the development of differentiated cell types. Antisense mutagenesis has shown that cells lacking CMF cannot aggregate, and preliminary data suggest that CMF regulates cAMP signal transduction. Calculations indicate that a mechanism of simultaneously secreting and recognizing a signal molecule, as used by Dictyostelium to monitor cell density, could also be used to determine the total number of cells in a tissue.

MeSH Terms
Animals Cell Adhesion Molecules/physiology Cyclic AMP/physiology Dictyostelium/genetics,growth & development Gene Expression Regulation Promoter Regions, Genetic Protozoan Proteins Signal Transduction
Chemicals
Cell Adhesion Molecules Protozoan Proteins cell cohesion molecule, Dictyostelium Cyclic AMP
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Clarke M
Oklahoma Medical Research Foundation, Program in Molecular and Cell Biology, Oklahoma City 73104, USA.
Gomer R H
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Article Info
Journal
Experientia
Abbr.
Experientia
ISSN
0014-4754
DOI
10.1007/BF01944730
Published
1995-12-18
Pages
1124-34
Language
English
Region
Switzerland
NLM ID
0376547
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01944730
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