Assignment of weight-based antibody units to a human antipneumococcal standard reference serum, lot 89-S.

Clinical and diagnostic laboratory immunology ·Vol. 2 ·No. 5 ·1995-09-00 ·Pages 590-7

Quataert SA, Kirch CS, Wiedl LJ, Phipps DC, Strohmeyer S, Cimino CO, Skuse J, Madore DV

Abstract

A human reference serum pool, lot 89-S, has been developed for use in quantitating concentrations of antibody to Streptococcus pneumoniae. Weight-based units have been assigned to antibodies to 11 pneumococcal polysaccharide (PnPs) serotypes (1, 3, 4, 5, 6B, 7F, 9V, 14, 18C, 19F, and 23F) by using enzyme-linked immunosorbent assay methodology and a human standard reference serum, USNRP IS 1644. The experimentally derived assignments for anti-PnPs antibodies of the immunoglobulin G (IgG), IgM, and IgA isotypes in lot 89-S correlate well to the separately determined immunoglobulin assignment. These assignments for this antipneumococcal standard serum were used to quantitate IgG, IgM, and IgA isotype levels and the total immunoglobulin level in pediatric samples from a pneumococcal conjugate vaccine trial. The data indicate that these assignments may be used to assess levels of antibody to PnPs serotypes in human serum.

MeSH Terms

Adult Antibodies, Bacterial/blood,chemistry Antibody Specificity Antigens, Bacterial/chemistry,immunology Bacterial Vaccines/standards Humans Immune Sera/chemistry Immunization/standards Infant Polysaccharides, Bacterial/chemistry,immunology Reference Standards Streptococcus pneumoniae/classification,immunology Vaccines, Conjugate/standards

Chemicals

Antibodies, Bacterial Antigens, Bacterial Bacterial Vaccines Immune Sera Polysaccharides, Bacterial Vaccines, Conjugate

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Quataert S A

Lederle-Praxis Biologicals, West Henrietta, New York 14586-9728, USA.

Kirch C S

Wiedl L J

Phipps D C

Strohmeyer S

Cimino C O

Skuse J

Madore D V

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15 references, click to expand

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Article Info

Journal

Clinical and diagnostic laboratory immunology

Abbr.

Clin Diagn Lab Immunol

ISSN

1071-412X

DOI

10.1128/cdli.2.5.590-597.1995

Published

1995-09-00

Pages

590-7

Language

English

Region

United States

NLM ID

9421292

PMCID

PMC170204

Subset

External Links

PubMed source DOI: 10.1128/cdli.2.5.590-597.1995 PMC Full Text

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Assignment of weight-based antibody units to a human antipneumococcal standard reference serum, lot 89-S.

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