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PMID: 8550191 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

H2O2 induces monocyte apoptosis and reduces viability of Mycobacterium avium-M. intracellulare within cultured human monocytes.

Infection and immunity ·Vol. 64 ·No. 2 ·1996-02-00 ·Pages 452-9

Laochumroonvorapong P, Paul S, Elkon KB, Kaplan G

Abstract

Mycobacterium avium-M. intracellulare, an intracellular parasite of mononuclear phagocytes, rarely causes disease in immunocompetent individuals. In contrast, in human immunodeficiency virus type 1-infected patients, M. avium-M. intracellulare can infect almost every tissue and organ. This suggests that immunocompetent individuals have a protective mechanism to control or prevent the infection. How mycobacterial may be killed by the host immune response is unclear. We have recently reported that induction of apoptosis of Mycobacterium bovis BCG-infected macrophages with ATP4- was associated with killing of the intracellular mycobacteria. In the present study, a long-term culture of M. avium-M. intracellulare-infected monocytes was used to further evaluate the interaction between M. avium-M. intracellulare and primary human monocytes. In our system, M. avium-M. intracellulare parasitized the human monocytes and appeared to replicate slowly over 14 days within the host cells. To examine the role of apoptotic mechanisms in survival or death of intracellular mycobacteria, M. avium-M. intracellulare-infected human monocytes were treated with a monoclonal antibody to Fas receptor (APO-1/CD95) or with various concentrations of H2O2. Although both of these exogenous agents induced monocyte apoptosis, optimal killing (65% reduction in CFU) of intracellular M. avium-M. intracellulare was observed only when M. avium-M. intracellulare-infected cells were treated with 10 mM H2O2. Fas-induced apoptosis did not affect M. avium-M. intracellulare viability. Our results suggest that not all stimuli of monocyte apoptosis induce killing of intracellular M. avium-M. intracellulare. Since release of H2O2 following phagocytosis of mycobacteria has been documented, H2O2-induced apoptotic death of M. avium-M. intracellulare-infected monocytes and its association with killing of the intracellular bacilli may be a physiological mechanism of host defense against M. avium-M. intracellulare.

MeSH Terms
Acquired Immunodeficiency Syndrome/immunology Apoptosis/drug effects Cells, Cultured Cycloheximide/pharmacology DNA/metabolism Humans Hydrogen Peroxide/pharmacology Monocytes/drug effects,microbiology Mycobacterium avium Complex/drug effects,growth & development,immunology Mycobacterium avium-intracellulare Infection/immunology Nitric Oxide/physiology fas Receptor/physiology
Chemicals
fas Receptor Nitric Oxide DNA Cycloheximide Hydrogen Peroxide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Laochumroonvorapong P
Laboratory of Cellular Physiology and Immunology, Rockefeller University, New York, New York, USA.
Paul S
Elkon K B
Kaplan G
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.64.2.452-459.1996
Published
1996-02-00
Pages
452-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC173785
Subset
IM
Grants
NIAID NIH HHS · AI-22616 · United States
External Links
PubMed source DOI: 10.1128/iai.64.2.452-459.1996 PMC Full Text View journal details
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