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PMID: 8550481 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differentiation of Serratia liquefaciens into swarm cells is controlled by the expression of the flhD master operon.

Journal of bacteriology ·Vol. 178 ·No. 2 ·1996-01-00 ·Pages 554-9

Eberl L, Christiansen G, Molin S, Givskov M

Abstract

The velocity with which a swarming colony of Serratia liquefaciens colonizes the surface of a suitable solid substratum was controlled by modulating the expression of the flhD master operon. In liquid medium, the stimulation of flhD expression resulted in filamentous, multinucleate, and hyperflagellated cells that were indistinguishable from swarm cells isolated from the edge of a swarm colony. Thus, expression of the flhD master operon appears to play a central role in the process of swarm cell differentiation.

MeSH Terms
Bacterial Proteins/genetics Culture Media DNA-Binding Proteins/genetics Enzyme Induction/drug effects Escherichia coli Proteins Flagella/physiology Gene Expression Regulation, Bacterial/physiology Isopropyl Thiogalactoside/pharmacology Operon/genetics Phospholipases A/metabolism Serratia/enzymology,genetics,physiology Trans-Activators/genetics beta-Galactosidase/biosynthesis,genetics
Chemicals
Bacterial Proteins Culture Media DNA-Binding Proteins Escherichia coli Proteins Trans-Activators flhD protein, E coli Isopropyl Thiogalactoside Phospholipases A beta-Galactosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Eberl L
Department of Microbiology, Technical University of Denmark, Lyngby, Denmark.
Christiansen G
Molin S
Givskov M
References (15)
15 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.2.554-559.1996
Published
1996-01-00
Pages
554-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177693
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.178.2.554-559.1996 PMC Full Text View journal details
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