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PMID: 8551622 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Efficient transactivation of the minute virus of mice P38 promoter requires upstream binding of NS1.

Journal of virology ·Vol. 70 ·No. 2 ·1996-02-00 ·Pages 834-42

Lorson C, Burger LR, Mouw M, Pintel DJ

Abstract

The P38 promoter of the autonomous parvovirus minute virus of mice is strongly transactivated by the nonstructural protein NS1, a sequence-specific DNA-binding protein. In the context of the complete viral genome, the only unique cis-acting signals required for P38 transactivation by NS1 are the proximal Sp1 site and the TATA element. In the absence of additional upstream sequences, a dependence upon the NS1 binding site within the transactivation response region is observed. Addition of synthetic NS1 binding sites to transactivation response region deletion mutants can restore the ability of NS1 to transactivate P38, and NS1 transactivation has been directly correlated to its ability to bind upstream of the P38 promoter.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Cell Line DNA, Viral Gene Expression Regulation, Viral Genome, Viral Mice Minute Virus of Mice/genetics,isolation & purification Molecular Sequence Data Promoter Regions, Genetic Protein Binding Sequence Deletion Sp1 Transcription Factor/metabolism TATA Box Transcriptional Activation Up-Regulation Viral Nonstructural Proteins/chemistry,metabolism
Chemicals
DNA, Viral NS1 protein, minute virus of mice Sp1 Transcription Factor Viral Nonstructural Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lorson C
Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, Columbia 65212, USA.
Burger L R
Mouw M
Pintel D J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.70.2.834-842.1996
Published
1996-02-00
Pages
834-42
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC189886
Subset
IM
Grants
NIAID NIH HHS · R01 AI21302 · United States
NIAID NIH HHS · T32 AI07276 · United States
External Links
PubMed source DOI: 10.1128/JVI.70.2.834-842.1996 PMC Full Text View journal details
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