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PMID: 8557026 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Crystal structure of the flavohemoglobin from Alcaligenes eutrophus at 1.75 A resolution.

The EMBO journal ·Vol. 14 ·No. 24 ·1995-12-15 ·Pages 6067-77

Ermler U, Siddiqui RA, Cramm R, Friedrich B

Abstract

The molecular structure of the flavohemoglobin from Alcaligenes eutrophus has been determined to a resolution of 1.75 A and refined to an R-factor of 19.6%. The protein comprises two fused modules: a heme binding module, which belongs to the globin family, and an FAD binding oxidoreductase module, which adopts a fold like ferredoxin reductase. The most striking deviation of the bacterial globin structure from those of other species is the movement of helix E in a way to provide more space in the vicinity of the distal heme binding site. A comparison with other members of the ferredoxin reductase family shows similar tertiary structures for the individual FAD and NAD binding domains but largely different interdomain orientations. The heme and FAD molecules approach each other to a minimal distance of 6.3 A and adopt an interplanar angle of 80 degrees. The electron transfer from FAD to heme occurs in a predominantly polar environment and may occur directly or be mediated by a water molecule.

MeSH Terms
Alcaligenes/chemistry,genetics,metabolism Amino Acid Sequence Bacterial Proteins/chemistry,genetics,metabolism Binding Sites Crystallography, X-Ray Electrochemistry Electron Transport Ferredoxin-NADP Reductase/chemistry,genetics,metabolism Flavin-Adenine Dinucleotide/metabolism Flavoproteins/chemistry Heme/chemistry,metabolism Hemeproteins/chemistry,genetics,metabolism Models, Molecular Molecular Sequence Data Molecular Structure NAD/metabolism Protein Conformation Protein Structure, Tertiary Sequence Homology, Amino Acid
Chemicals
Bacterial Proteins Flavoproteins Hemeproteins flavohemoprotein, Bacteria NAD Flavin-Adenine Dinucleotide Heme Ferredoxin-NADP Reductase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ermler U
Max-Planck-Institut fur Biophysik, Frankfurt, Germany.
Siddiqui R A
Cramm R
Friedrich B
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-12-15
Pages
6067-77
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394731
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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