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PMID: 8576049 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Analysis of the Pseudomonas aeruginosa elastase (lasB) regulatory region.

Journal of bacteriology ·Vol. 178 ·No. 4 ·1996-02-00 ·Pages 1134-40

Rust L, Pesci EC, Iglewski BH

Abstract

The enzyme elastase is an important virulence factor of the opportunistic human pathogen Pseudomonas aeruginosa. Previous studies have shown that expression of the P. aeruginosa elastase gene (lasB) requires both an activator protein, LasR, and an N-acylhomoserine lactone compound termed Pseudomonas autoinducer (PAI). In this study, we analyzed the lasB promoter region to learn more about lasB activation by LasR and PAI. We report that the lasB transcriptional start is located 141 nucleotides upstream from the lasB translational start. It was also discovered that the lasB promoter region contains two putative operator sequences (OP1 and OP2) that are similar to each other and the Vibrio fischeri lux operator. OP1 is located directly upstream from, and may overlap with, the lasB promoter region, and OP2 is centered 102 nucleotides upstream from the lasB transcriptional start site. To study the effects of these putative operators and other sequences upstream from the lasB transcriptional start site on lasB activation, a series of transcriptional lasBp-lacZ gene fusions was constructed. Data from these fusions indicate that both putative operators are involved in LasR- and PAI-mediated lasB activation, with OP1 being more important than OP2.

MeSH Terms
Bacterial Proteins/genetics Base Sequence DNA-Binding Proteins/metabolism Enzyme Induction Gene Expression Regulation, Bacterial Homoserine/analogs & derivatives,metabolism Lactones/metabolism Metalloendopeptidases/genetics Molecular Sequence Data Mutagenesis, Site-Directed Operator Regions, Genetic Pancreatic Elastase/genetics Promoter Regions, Genetic Pseudomonas aeruginosa/enzymology,genetics Regulatory Sequences, Nucleic Acid Sequence Deletion Trans-Activators/metabolism Transcription, Genetic
Chemicals
Bacterial Proteins DNA-Binding Proteins Lactones LasR protein, Pseudomonas aeruginosa Trans-Activators Pseudomonas aeruginosa autoinducer Homoserine Pancreatic Elastase Metalloendopeptidases pseudolysin, Pseudomonas aeruginosa
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rust L
Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, New York 14642, USA.
Pesci E C
Iglewski B H
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.4.1134-1140.1996
Published
1996-02-00
Pages
1134-40
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177776
Subset
IM
Grants
NIAID NIH HHS · 1-RO1-AI33713 · United States
NIAID NIH HHS · 5-T32-AI07362 · United States
Databases
GENBANK
M19472
External Links
PubMed source DOI: 10.1128/jb.178.4.1134-1140.1996 PMC Full Text View journal details
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