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PMID: 8576343 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Analysis of Enterococcus faecalis isolates from intercontinental sources by multilocus enzyme electrophoresis and pulsed-field gel electrophoresis.

Journal of clinical microbiology ·Vol. 33 ·No. 11 ·1995-11-00 ·Pages 2903-7

Tomayko JF, Murray BE

Abstract

Pulsed-field gel electrophoresis (PFGE) and multilocus enzyme electrophoresis (MLEE) were compared in this study of 65 Enterococcus faecalis isolates recovered over a 20-year period from diverse geographic sources. Clonal relationships recognized by PFGE were also recognized by MLEE; however, MLEE recognized as greater number of isolates as belonging to clonal groups than did PFGE. Both techniques were reproducible and discriminatory, but MLEE more readily recognized relationships among large numbers of isolates. MLEE confirmed the previously reported clonal spread of beta-lactamase-producing E. faecalis to six hospitals in five states. MLEE provided a useful population framework of the E. faecalis isolates in this sample, while PFGE was able to differentiate among isolates within some MLEE clonal groups.

MeSH Terms

Alleles Cross Infection DNA, Bacterial/analysis Electrophoresis, Gel, Pulsed-Field Enterococcus faecalis/classification,enzymology,genetics Enzymes/analysis,genetics Gram-Positive Bacterial Infections/epidemiology Humans

Chemicals

DNA, Bacterial Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Tomayko J F

Department of Internal Medicine, University of Texas Medical School, Houston 77030, USA.

Murray B E

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20 references, click to expand

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.33.11.2903-2907.1995

Published

1995-11-00

Pages

2903-7

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC228604

Subset

External Links

PubMed source DOI: 10.1128/jcm.33.11.2903-2907.1995 PMC Full Text View journal details

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Analysis of Enterococcus faecalis isolates from intercontinental sources by multilocus enzyme electrophoresis and pulsed-field gel electrophoresis.

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