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PMID: 8577712 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sterol regulation of acetyl coenzyme A carboxylase: a mechanism for coordinate control of cellular lipid.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 3 ·1996-02-06 ·Pages 1049-53

Lopez JM, Bennett MK, Sanchez HB, Rosenfeld JM, Osborne TF

Abstract

Transcription from the housekeeping promoter for the acetyl coenzyme A carboxylase (ACC) gene, which encodes the rate-controlling enzyme of fatty acid biosynthesis, is shown to be regulated by cellular sterol levels through novel binding sites for the sterol-sensitive sterol regulatory element binding protein (SREBP)-1 transcription factor. The position of the SREBP sites relative to those for the ubiquitous auxiliary transcription factor Sp1 is reminiscent of that previously described for the sterol-regulated low density lipoprotein receptor promoter. The experiments provide molecular evidence that the metabolism of fatty acids and cholesterol, two different classes of essential cellular lipids, are coordinately regulated by cellular lipid levels.

MeSH Terms

Acetyl-CoA Carboxylase/biosynthesis,genetics Animals Base Sequence Binding Sites CCAAT-Enhancer-Binding Proteins Cell Line Cholesterol/metabolism Cloning, Molecular DNA Footprinting DNA, Complementary DNA-Binding Proteins/metabolism Deoxyribonuclease I Fatty Acids/metabolism Gene Expression Regulation, Enzymologic Helix-Loop-Helix Motifs Homeostasis Humans Kinetics Lipid Metabolism Molecular Sequence Data Nuclear Proteins/metabolism Polymerase Chain Reaction Promoter Regions, Genetic Rats Recombinant Proteins/biosynthesis Sterol Regulatory Element Binding Protein 1 Sterols/biosynthesis TATA Box Transcription Factors/metabolism Transcription, Genetic Transfection Tumor Cells, Cultured

Chemicals

CCAAT-Enhancer-Binding Proteins DNA, Complementary DNA-Binding Proteins Fatty Acids Nuclear Proteins Recombinant Proteins SREBF1 protein, human Srebf1 protein, rat Sterol Regulatory Element Binding Protein 1 Sterols Transcription Factors Cholesterol Deoxyribonuclease I Acetyl-CoA Carboxylase

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Lopez J M

Department of Molecular Biology and Biochemistry, University of California, Irvine 92717, USA.

Bennett M K

Sanchez H B

Rosenfeld J M

Osborne T F

References (15)

15 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.93.3.1049

Published

1996-02-06

Pages

1049-53

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC40028

Subset

Grants

NHLBI NIH HHS · HL48044 · United States

External Links

PubMed source DOI: 10.1073/pnas.93.3.1049 PMC Full Text View journal details

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Sterol regulation of acetyl coenzyme A carboxylase: a mechanism for coordinate control of cellular lipid.

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