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PMID: 8582634 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Roles of ABF1, NPL3, and YCL54 in silencing in Saccharomyces cerevisiae.

Genetics ·Vol. 141 ·No. 3 ·1995-11-00 ·Pages 889-902

Loo S, Laurenson P, Foss M, Dillin A, Rine J

Abstract

A sensitized genetic screen was carried out to identify essential genes involved in silencing in Saccharomyces cerevisiae. This screen identified temperature-sensitive alleles of ORC2 and ORC5, as described elsewhere, and ABF1, NPL3, and YCL54, as described here. Alleles of ABF1 that caused silencing defects provided the genetic proof of Abflp's role in silencing. The roles of Npl3p and Ycl54p are less clear. These proteins did not act exclusively through any one of the three protein binding sites of the HMR-E silencer. Unlike the orc2, orc5, and abf1 mutations that were isolated in the same (or a similar) screen for silencing mutants, neither temperature-sensitive mutation in NPL3 or YCL54 caused overt replication defects.

MeSH Terms
Alleles Amino Acid Sequence Cell Cycle DNA-Binding Proteins/genetics,physiology Escherichia coli/genetics Fungal Proteins/genetics,physiology Gene Expression Regulation, Fungal Mating Factor Methyltransferases Molecular Sequence Data Mutagenesis Nuclear Proteins/genetics,physiology Peptides/genetics RNA-Binding Proteins Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics,growth & development Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Homology, Amino Acid Temperature Transcription Factors/genetics,physiology Transcription, Genetic
Chemicals
ABF1 protein, S cerevisiae DNA-Binding Proteins Fungal Proteins NPL3 protein, S cerevisiae Nuclear Proteins Peptides RNA-Binding Proteins Saccharomyces cerevisiae Proteins Transcription Factors Mating Factor Methyltransferases SPB1 protein, S cerevisiae
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Loo S
Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
Laurenson P
Foss M
Dillin A
Rine J
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/141.3.889
Published
1995-11-00
Pages
889-902
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1206852
Subset
IM
Grants
NIGMS NIH HHS · GM-31105 · United States
NIEHS NIH HHS · P30ESO1896-12 · United States
External Links
PubMed source DOI: 10.1093/genetics/141.3.889 PMC Full Text View journal details
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