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PMID: 8584934 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Role of Rho in chemoattractant-activated leukocyte adhesion through integrins.

Science (New York, N.Y.) ·Vol. 271 ·No. 5251 ·1996-02-16 ·Pages 981-3

Laudanna C, Campbell JJ, Butcher EC

Abstract

Heterotrimeric guanine nucleotide binding protein (G protein)-linked receptors of the chemoattractant subfamily can trigger adhesion through leukocyte integrins, and in this role they are thought to regulate immune cell-cell interactions and trafficking. In lymphoid cells transfected with formyl peptide or interleukin-8 receptors, agonist stimulation activated nucleotide exchange on the small guanosine triphosphate-binding protein RhoA in seconds. Inactivation of Rho by C3 transferase exoenzyme blocked agonist-induced lymphocyte alpha4beta1 adhesion to vascular cell adhesion molecule-1 and neutrophil beta2 integrin adhesion to fibrinogen. These findings suggest that Rho participates in signaling from chemoattractant receptors to trigger rapid adhesion in leukocytes.

MeSH Terms
Amino Acid Sequence Animals Antigens, CD/genetics B-Lymphocytes/physiology Cell Adhesion Cells, Cultured Chemotactic Factors/pharmacology GTP-Binding Proteins/metabolism,physiology Guanosine 5'-O-(3-Thiotriphosphate)/metabolism Guanosine Diphosphate/metabolism Integrin alpha4beta1 Integrins/physiology Interleukin-8/pharmacology Mice Molecular Sequence Data N-Formylmethionine Leucyl-Phenylalanine/pharmacology Receptors, Formyl Peptide Receptors, Immunologic/genetics Receptors, Interleukin/genetics Receptors, Interleukin-8A Receptors, Lymphocyte Homing/physiology Receptors, Peptide/genetics Signal Transduction Tetradecanoylphorbol Acetate/pharmacology Transfection Vascular Cell Adhesion Molecule-1/physiology rhoA GTP-Binding Protein
Chemicals
Antigens, CD Chemotactic Factors Integrin alpha4beta1 Integrins Interleukin-8 Receptors, Formyl Peptide Receptors, Immunologic Receptors, Interleukin Receptors, Interleukin-8A Receptors, Lymphocyte Homing Receptors, Peptide Vascular Cell Adhesion Molecule-1 Guanosine Diphosphate Guanosine 5'-O-(3-Thiotriphosphate) N-Formylmethionine Leucyl-Phenylalanine GTP-Binding Proteins rhoA GTP-Binding Protein Tetradecanoylphorbol Acetate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Laudanna C
Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University, CA 94305, USA.
Campbell J J
Butcher E C
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.271.5251.981
Published
1996-02-16
Pages
981-3
Language
English
Region
United States
NLM ID
0404511
Subset
IM
Grants
NIAID NIH HHS · 1F32 AI08930 · United States
NCI NIH HHS · 5T32 CA09302 · United States
External Links
PubMed source DOI: 10.1126/science.271.5251.981 View journal details
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