Disease resistance conferred by expression of a gene encoding H2O2-generating glucose oxidase in transgenic potato plants.

The Plant cell ·Vol. 7 ·No. 9 ·1995-09-00 ·Pages 1357-68

Wu G, Shortt BJ, Lawrence EB, Levine EB, Fitzsimmons KC, Shah DM

Abstract

Plant defense responses to pathogen infection involve the production of active oxygen species, including hydrogen peroxide (H2O2). We obtained transgenic potato plants expressing a fungal gene encoding glucose oxidase, which generates H2O2 when glucose is oxidized. H2O2 levels were elevated in both leaf and tuber tissues of these plants. Transgenic potato tubers exhibited strong resistance to a bacterial soft rot disease caused by Erwinia carotovora subsp carotovora, and disease resistance was sustained under both aerobic and anaerobic conditions of bacterial infection. This resistance to soft rot was apparently mediated by elevated levels of H2O2, because the resistance could be counteracted by exogenously added H2O2-degrading catalase. The transgenic plants with increased levels of H2O2 also exhibited enhanced resistance to potato late blight caused by Phytophthora infestans. The development of lesions resulting from infection by P. infestans was significantly delayed in leaves of these plants. Thus, the expression of an active oxygen species-generating enzyme in transgenic plants represents a novel approach for engineering broad-spectrum disease resistance in plants.

MeSH Terms

Base Sequence DNA Primers Gene Expression Glucose Oxidase/genetics Hydrogen Peroxide/metabolism Molecular Sequence Data Pectobacterium carotovorum/growth & development,pathogenicity Phytophthora/growth & development,pathogenicity Plant Diseases/microbiology Plants, Genetically Modified Plants, Toxic Tobacco/enzymology,genetics,immunology,metabolism

Chemicals

DNA Primers Hydrogen Peroxide Glucose Oxidase

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Wu G

Monsanto Company, St. Louis, Missouri 63198, USA.

Shortt B J

Lawrence E B

Levine E B

Fitzsimmons K C

Shah D M

References (24)

24 references, click to expand

Rapid Stimulation of an Oxidative Burst during Elicitation of Cultured Plant Cells : Role in Defense and Signal Transduction.
Plant Physiol. 1989 May;90(1):109-16 PMID: 16666719
Involvement of Oxidative Processes in the Signaling Mechanisms Leading to the Activation of Glyceollin Synthesis in Soybean (Glycine max).
Plant Physiol. 1994 Mar;104(3):945-952 PMID: 12232139
Abrupt increase in the level of hydrogen peroxide in leaves of winter wheat is caused by cold treatment.
Plant Physiol. 1991 Nov;97(3):1265-7 PMID: 16668520
Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL-3.
FEBS Lett. 1989 Sep 11;255(1):63-6 PMID: 2792372
Characteristics of a strong promoter from figwort mosaic virus: comparison with the analogous 35S promoter from cauliflower mosaic virus and the regulated mannopine synthase promoter.
Plant Mol Biol. 1990 Mar;14(3):433-43 PMID: 2102823
Generation of superoxide anion and hydrogen peroxide at the surface of plant cells.
J Bioenerg Biomembr. 1991 Jun;23(3):409-23 PMID: 1650779
Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: a novel, rapid defense response.
Cell. 1992 Jul 10;70(1):21-30 PMID: 1623521
The salicylic acid signal in plants.
Plant Mol Biol. 1994 Dec;26(5):1439-58 PMID: 7858199
The molecular biology of disease resistance.
Plant Mol Biol. 1992 May;19(1):109-22 PMID: 1600163
Apoplastic expression of yeast-derived invertase in potato : effects on photosynthesis, leaf solute composition, water relations, and tuber composition.
Plant Physiol. 1992 Sep;100(1):301-8 PMID: 16652961
Sequence of figwort mosaic virus DNA (caulimovirus group).
Nucleic Acids Res. 1987 Oct 26;15(20):8451-66 PMID: 3671088
Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid.
Science. 1993 Dec 17;262(5141):1883-6 PMID: 8266079
Activation, structure, and organization of genes involved in microbial defense in plants.
Adv Genet. 1990;28:165-234 PMID: 2239449
Sterol ester hydrolase from rat pancreas.
Methods Enzymol. 1981;71 Pt C:664-74 PMID: 7278675
Active Oxygen Species in Plant Defense against Pathogens.
Plant Physiol. 1994 Jun;105(2):467-472 PMID: 12232215
H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response.
Cell. 1994 Nov 18;79(4):583-93 PMID: 7954825
Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-kappa B transcription factor and HIV-1.
EMBO J. 1991 Aug;10(8):2247-58 PMID: 2065663
Involvement of hydrogen peroxide in the regulation of senescence in pear.
Plant Physiol. 1977 Mar;59(3):411-6 PMID: 16659863
Defense-related proteins in higher plants.
Annu Rev Biochem. 1990;59:873-907 PMID: 2197993
Signals and transduction mechanisms for activation of plant defenses against microbial attack.
Cell. 1989 Jan 27;56(2):215-24 PMID: 2643475
Fungal Elicitor-Induced Bean Proline-Rich Protein mRNA Down-Regulation Is Due to Destabilization That Is Transcription and Translation Dependent.
Plant Cell. 1993 Sep;5(9):1089-1099 PMID: 12271097
Function of Oxidative Cross-Linking of Cell Wall Structural Proteins in Plant Disease Resistance.
Plant Cell. 1994 Dec;6(12):1703-1712 PMID: 12244231
Plasma Membrane Redox Enzyme Is Involved in the Synthesis of O2- and H2O2 by Phytophthora Elicitor-Stimulated Rose Cells.
Plant Physiol. 1995 Apr;107(4):1241-1247 PMID: 12228430
Osmotin overexpression in potato delays development of disease symptoms.
Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1888-92 PMID: 11607463

Article Info

Journal

The Plant cell

Abbr.

Plant Cell

ISSN

1040-4651

DOI

10.1105/tpc.7.9.1357

Published

1995-09-00

Pages

1357-68

Language

English

Region

England

NLM ID

9208688

PMCID

PMC160957

Subset

External Links

PubMed source DOI: 10.1105/tpc.7.9.1357 PMC Full Text View journal details

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Disease resistance conferred by expression of a gene encoding H2O2-generating glucose oxidase in transgenic potato plants.

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