Chlamydia trachomatis interrupts an exocytic pathway to acquire endogenously synthesized sphingomyelin in transit from the Golgi apparatus to the plasma membrane.

The EMBO journal ·Vol. 15 ·No. 5 ·1996-03-01 ·Pages 964-77

Hackstadt T, Rockey DD, Heinzen RA, Scidmore MA

Abstract

Chlamydia trachomatis acquires C6-NBD-sphingomyelin endogenously synthesized from C6-NBD-ceramide and transported to the vesicle (inclusion) in which they multiply. Here we explore the mechanisms of this unusual trafficking and further characterize the association of the chlamydial inclusion with the Golgi apparatus. Endocytosed chlamydiae are trafficked to the Golgi region and begin to acquire sphingolipids from the host within a few hours following infection. The transport of NBD-sphingolipid to the inclusion is energy- and temperature-dependent with the characteristics of an active, vesicle-mediated process. Photo-oxidation of C5-DMB-ceramide, in the presence of diaminobenzidine, identified DMB-lipids in vesicles in the process of fusing to the chlamydial inclusion membrane. C6-NBD-sphingomyelin incorporated into the plasma membrane is not trafficked to the inclusion to a significant degree, suggesting the pathway for sphingomyelin trafficking is direct from the Golgi apparatus to the chlamydial inclusion. Lectins and antibody probes for Golgi-specific glycoproteins demonstrate the close association of the chlamydial inclusion with the Golgi apparatus but do not detect these markers in the inclusion membrane. Collectively, the data are consistent with a model in which C.trachomatis inhabits a unique vesicle which interrupts an exocytic pathway to intercept host sphingolipids in transit from the Golgi apparatus to the plasma membrane.

MeSH Terms

4-Chloro-7-nitrobenzofurazan/analogs & derivatives,metabolism Animals Anti-Bacterial Agents/pharmacology Biological Transport, Active Brefeldin A Cell Membrane/metabolism Ceramides/metabolism Chlamydia Infections/metabolism,microbiology,pathology Chlamydia trachomatis/drug effects,pathogenicity,physiology Cyclopentanes/pharmacology Exocytosis Fluorescent Dyes/metabolism Golgi Apparatus/metabolism HeLa Cells Humans Inclusion Bodies/drug effects,metabolism,microbiology Macrolides Membrane Fusion Microscopy, Electron Models, Biological Sphingomyelins/metabolism

Chemicals

Anti-Bacterial Agents Ceramides Cyclopentanes Fluorescent Dyes Macrolides N-4-nitrobenzo-2-oxa-1,3-diazoleaminocaproyl sphingosylphosphorylcholine Sphingomyelins Brefeldin A N-(7-(4-nitrobenzo-2-oxa-1,3-diazole))-6-aminocaproyl sphingosine 4-Chloro-7-nitrobenzofurazan

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Hackstadt T

Laboratory of Intracellular Parasites, Rock Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USA.

Rockey D D

Heinzen R A

Scidmore M A

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1996-03-01

Pages

964-77

Language

English

Region

England

NLM ID

8208664

PMCID

PMC449991

Subset

External Links

PubMed source PMC Full Text View journal details

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Chlamydia trachomatis interrupts an exocytic pathway to acquire endogenously synthesized sphingomyelin in transit from the Golgi apparatus to the plasma membrane.

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