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PMID: 8616213 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Atypical phytochrome gene structure in the green alga Mesotaenium caldariorum.

Plant molecular biology ·Vol. 29 ·No. 6 ·1995-12-00 ·Pages 1127-42

Lagarias DM, Wu SH, Lagarias JC

Abstract

The phytochrome photoreceptor in the green alga Mesotaenium caldariorum is encoded by a small family of highly related genes. DNA sequence analysis of two of the algal phytochrome genes indicates an atypical gene structure with numerous long introns. The two genes, termed mesphy1a and mesphy1b, encode polypeptides which differ by one amino acid in the region of overlap that was sequenced. RT-PCR studies have established the intron-exon junctions of both genes and show that both are expressed. RNA blot analysis indicates a single transcript of ca. 4.1 kb in length. The deduced amino acid sequence of the mesphy1b gene reveals that the photoreceptor consists of 1142 amino acids, with an overall structure similar to other phytochromes. Phylogenetic analyses indicate that the algal phytochrome falls into a distinct subfamily with other lower plant phytochromes. Profile analysis of an internal repeat found within the central hinge region of the phytochrome polypeptide indicates an evolutionary relatedness to the photoactive yellow protein from the purple bacterium Ectothiorhodospira halophila, to several bacterial sensor kinase family members, and to a family of eukaryotic regulatory proteins which includes the period clock (per) and single-minded (sim) gene products of Drosophila. Since mutations which alter phytochrome activity cluster within the region delimited by these direct repeats (P.H. Quail et al., Science 268 (1995): 675-680), this conserved motif may play an important role in the signal transducing function of these disparate protein families.

MeSH Terms
Amino Acid Sequence Animals Bacteria/genetics Base Sequence Basic Helix-Loop-Helix Transcription Factors Biological Evolution Chlorophyta/genetics Consensus Sequence DNA Primers DNA-Binding Proteins/genetics Drosophila/genetics Drosophila Proteins Exons Genes, Plant Introns Molecular Sequence Data Nuclear Proteins/genetics Period Circadian Proteins Phylogeny Phytochrome/genetics Polymerase Chain Reaction/methods Repetitive Sequences, Nucleic Acid Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid
Chemicals
Basic Helix-Loop-Helix Transcription Factors DNA Primers DNA-Binding Proteins Drosophila Proteins Nuclear Proteins PER protein, Drosophila Period Circadian Proteins sim protein, Drosophila Phytochrome
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lagarias D M
Section of Molecular and Cellular Biology, University of California, Davis 95616, USA.
Wu S H
Lagarias J C
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00020457
Published
1995-12-00
Pages
1127-42
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Grants
NCRR NIH HHS · 1 P41 RR06009 · United States
Databases
GENBANK
D13519, L19596, L29642, M31067, U10325, U31283, U31284, X67684, Z22637
SWISSPROT
P05709, P08400, P10931, P10955, P13260, P16113, P25764, P26167, P26762, P29130, P30663, P33530, P37739, P39764, Q03353, Q04855, Q06067
External Links
PubMed source DOI: 10.1007/BF00020457 View journal details
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