Nucleotide sequence and characterization of erythromycin resistance determinant that encodes macrolide 2'-phosphotransferase I in Escherichia coli.

Antimicrobial agents and chemotherapy ·Vol. 39 ·No. 10 ·1995-10-00 ·Pages 2359-63

Noguchi N, Emura A, Matsuyama H, O'Hara K, Sasatsu M, Kono M

Abstract

The DNA fragment (3.3 kb) containing the erythromycin resistance determinant was cloned from Escherichia coli Tf481A and sequenced. Deletion and complementation analyses indicated that the expression of high-level resistance to erythromycin requires two genes, mphA and mrx, which encode macrolide 2'-phosphotransferase I and an unidentified hydrophobic protein, respectively.

MeSH Terms

Amino Acid Sequence Anti-Bacterial Agents/metabolism,pharmacology Base Sequence Drug Resistance, Microbial/genetics Erythromycin/pharmacology Escherichia coli/enzymology Molecular Sequence Data Open Reading Frames Phosphotransferases/genetics Phosphotransferases (Alcohol Group Acceptor)/genetics

Chemicals

Anti-Bacterial Agents Erythromycin Phosphotransferases Phosphotransferases (Alcohol Group Acceptor) macrolide 2'-kinase

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Noguchi N

Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.

Emura A

Matsuyama H

O'Hara K

Sasatsu M

Kono M

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16 references, click to expand

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Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.39.10.2359

Published

1995-10-00

Pages

2359-63

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC162946

Subset

Databases

GENBANK

D16251

External Links

PubMed source DOI: 10.1128/AAC.39.10.2359 PMC Full Text View journal details

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Nucleotide sequence and characterization of erythromycin resistance determinant that encodes macrolide 2'-phosphotransferase I in Escherichia coli.

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