Cloning of a putative ligand for the T1/ST2 receptor.

The Journal of biological chemistry ·Vol. 271 ·No. 10 ·1996-03-08 ·Pages 5784-9

Gayle MA, Slack JL, Bonnert TP, Renshaw BR, Sonoda G, Taguchi T, Testa JR, Dower SK, Sims JE

Abstract

T1/ST2 is a receptor-like molecule homologous to the type I interleukin-1 receptor. Despite this sequence similarity, we have been unable to demonstrate binding of T1/ST2 to any of the three interleukin-1 species. In searching for a ligand for T1/ST2, we have cloned a cell surface protein to which it binds. This protein is unable to initiate signal transduction by the T1/ST2 receptor in several in vitro assays.

MeSH Terms

3T3 Cells Amino Acid Sequence Animals Cell Line Cloning, Molecular DNA/metabolism Female Humans Interleukin-1 Receptor-Like 1 Protein Interleukin-8/genetics Kinetics Membrane Proteins Mice Molecular Sequence Data NF-kappa B/metabolism Organ Specificity Promoter Regions, Genetic Protein Biosynthesis Proteins/metabolism Rats Receptors, Cell Surface Receptors, Interleukin Receptors, Interleukin-1/biosynthesis,chemistry,metabolism Recombinant Proteins/biosynthesis,chemistry,metabolism Sequence Homology, Amino Acid Substrate Specificity Tumor Cells, Cultured

Chemicals

IL1RL1 protein, human Il1rl1 protein, mouse Interleukin-1 Receptor-Like 1 Protein Interleukin-8 Membrane Proteins NF-kappa B Proteins Receptors, Cell Surface Receptors, Interleukin Receptors, Interleukin-1 Recombinant Proteins DNA

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Gayle M A

Immunex Corporation, Seattle, Washington 98101, USA.

Slack J L

Bonnert T P

Renshaw B R

Sonoda G

Taguchi T

Testa J R

Dower S K

Sims J E

Article Info

Journal

The Journal of biological chemistry

Abbr.

J Biol Chem

ISSN

0021-9258

DOI

10.1074/jbc.271.10.5784

Published

1996-03-08

Pages

5784-9

Language

English

Region

United States

NLM ID

2985121R

Subset

Databases

GENBANK

U41804, U41805

External Links

PubMed source DOI: 10.1074/jbc.271.10.5784 View journal details

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Cloning of a putative ligand for the T1/ST2 receptor.

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