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PMID: 8622889 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Delay in serum stimulation of Erk activity caused by oncogenic transformation.

Oncogene ·Vol. 12 ·No. 8 ·1996-04-18 ·Pages 1689-95

Greulich H, Reichman C, Hanafusa H

Abstract

Mitogenic growth factor stimulation activates several signal transduction pathways, including the well-characterized Ras-Erk pathway, resulting in transient activation of Erk1 and Erk2. Oncogenic transformation, however, causes constitutive activation of growth signalling pathways, resulting in an accelerated rate of cell division. We investigated the effects of transformation on serum and growth factor stimulation of Erk1 and Erk2, and show that stimulation of these MAP kinases, as well as the Erk activator Mek, is delayed in oncogene transformed cells. Possible mechanisms of this delay are explored. In addition, our data indicate that prolonged mitogenic stimulation does not necessarily result in constitutive activation of Erk1 and Erk2.

MeSH Terms

Animals Calcium-Calmodulin-Dependent Protein Kinases/drug effects,genetics,metabolism Cell Cycle Proteins Cell Line, Transformed Cell Transformation, Neoplastic Chick Embryo Culture Media, Serum-Free Dual Specificity Phosphatase 1 Enzyme Activation/drug effects,genetics ErbB Receptors/metabolism Fibroblasts Genes, ras Immediate-Early Proteins/genetics,metabolism Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinase Kinases Mitogen-Activated Protein Kinases Mutation Oncogene Protein v-crk Phosphoprotein Phosphatases Phosphorylation Protein Kinases/metabolism Protein Phosphatase 1 Protein Tyrosine Phosphatases/genetics,metabolism Protein-Tyrosine Kinases/drug effects,genetics,metabolism Rats Retroviridae Proteins, Oncogenic/genetics Signal Transduction Time Factors

Chemicals

Cell Cycle Proteins Culture Media, Serum-Free Immediate-Early Proteins Oncogene Protein v-crk Retroviridae Proteins, Oncogenic Protein Kinases ErbB Receptors Protein-Tyrosine Kinases Calcium-Calmodulin-Dependent Protein Kinases Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinases Mitogen-Activated Protein Kinase Kinases Phosphoprotein Phosphatases Protein Phosphatase 1 Dual Specificity Phosphatase 1 Dusp1 protein, rat Protein Tyrosine Phosphatases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Greulich H

Laboratory of Molecular Oncology, Rockefeller University, New York, NY 10021, USA.

Reichman C

Hanafusa H

Article Info

Journal

Oncogene

Abbr.

Oncogene

ISSN

0950-9232

Published

1996-04-18

Pages

1689-95

Language

English

Region

England

NLM ID

8711562

Subset

Grants

NCI NIH HHS · CA09673-19 · United States

NCI NIH HHS · CA44356 · United States

External Links

PubMed source View journal details

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Delay in serum stimulation of Erk activity caused by oncogenic transformation.

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