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PMID: 8625408 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Asymmetric accumulation of Ash1p in postanaphase nuclei depends on a myosin and restricts yeast mating-type switching to mother cells.

Cell ·Vol. 84 ·No. 5 ·1996-03-08 ·Pages 699-709

Bobola N, Jansen RP, Shin TH, Nasmyth K

Abstract

Cell division in haploid yeast gives rise to a "mother" cell capable of mating-type switching and a "daughter" cell that is not. Switching is initiated by the HO endonuclease, whose gene is only transcribed in cells that have previously given birth to a bud (mother cells). HO expression depends on a minimyosin, She1p/Myo4p, which accumulates preferentially in growing buds. We describe a gene, ASH1, that is necessary to repress HO in daughters. ASH1 encodes a zinc finger protein whose preferential accumulation in daughter cell nuclei at the end of anaphase depends on She1p/Myo4p. The greater abundance of Ash1p in daughter cells is responsible for restricting HO expression to mother cells.

MeSH Terms

Amino Acid Sequence Anaphase Base Sequence Cell Nucleus/metabolism Cloning, Molecular Crosses, Genetic DNA-Binding Proteins Deoxyribonucleases, Type II Site-Specific/biosynthesis,genetics Epitopes/analysis Fungal Proteins/biosynthesis,metabolism Gene Expression Genes, Fungal Genes, Mating Type, Fungal Molecular Sequence Data Mutagenesis Myosin Heavy Chains Myosin Type V Myosins/biosynthesis,metabolism Open Reading Frames Recombinant Proteins/biosynthesis,chemistry Repressor Proteins Saccharomyces cerevisiae/cytology,genetics,physiology Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Species Specificity Transcription Factors/biosynthesis,chemistry,genetics Transcription, Genetic Zinc Fingers

Chemicals

ASH1 protein, S cerevisiae DNA-Binding Proteins Epitopes Fungal Proteins Myo4 protein, S cerevisiae Recombinant Proteins Repressor Proteins Saccharomyces cerevisiae Proteins Transcription Factors HO protein, S cerevisiae SCEI protein, S cerevisiae Deoxyribonucleases, Type II Site-Specific Myosin Type V Myosin Heavy Chains Myosins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Bobola N

Research Institute of Molecular Pathology, Vienna, Austria.

Jansen R P

Shin T H

Nasmyth K

Article Info

Journal

Cell

Abbr.

Cell

ISSN

0092-8674

DOI

10.1016/s0092-8674(00)81048-x

Published

1996-03-08

Pages

699-709

Language

English

Region

United States

NLM ID

0413066

Subset

Corrections

CommentIn

PMID: 8625401

External Links

PubMed source DOI: 10.1016/s0092-8674(00)81048-x View journal details

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Asymmetric accumulation of Ash1p in postanaphase nuclei depends on a myosin and restricts yeast mating-type switching to mother cells.

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