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PMID: 8626344 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Integration host factor amplifies the induction of the aceBAK operon of Escherichia coli by relieving IclR repression.

Journal of bacteriology ·Vol. 178 ·No. 9 ·1996-05-00 ·Pages 2715-7

Resnik E, Pan B, Ramani N, Freundlich M, LaPorte DC

Abstract

A binding site for integration host factor (IHF) was identified upstream of the aceBAK promoter. Under inducing conditions, IHF activates aceB::lacZ expression by opposing IclR repression. In contrast, IHF has little effect on aceB::lacZ expression under repressing conditions. The ability of IHF to relieve repression under inducing but not repressing conditions allows this protein to amplify the induction of aceBAK.

MeSH Terms
Acetates/metabolism Bacterial Proteins/metabolism,physiology Base Sequence Binding Sites DNA, Bacterial/metabolism DNA, Recombinant DNA-Binding Proteins/metabolism Enzyme Induction Enzyme Repression Escherichia coli/genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial/physiology Glucose/metabolism Integration Host Factors Molecular Sequence Data Operon Promoter Regions, Genetic/genetics Repressor Proteins/physiology Transcription Factors beta-Galactosidase/genetics
Chemicals
Acetates Bacterial Proteins DNA, Bacterial DNA, Recombinant DNA-Binding Proteins Escherichia coli Proteins Integration Host Factors Repressor Proteins Transcription Factors IclR protein, E coli beta-Galactosidase Glucose
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Resnik E
Department of Biochemistry, University of Minnesota, Minneapolis 55455, USA.
Pan B
Ramani N
Freundlich M
LaPorte D C
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20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.9.2715-2717.1996
Published
1996-05-00
Pages
2715-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC178001
Subset
IM
Grants
NIDDK NIH HHS · DK40486 · United States
NIGMS NIH HHS · GM17152 · United States
External Links
PubMed source DOI: 10.1128/jb.178.9.2715-2717.1996 PMC Full Text View journal details
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