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PMID: 8629022 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor.

Science (New York, N.Y.) ·Vol. 272 ·No. 5263 ·1996-05-10 ·Pages 872-7

Feng Y, Broder CC, Kennedy PE, Berger EA

Abstract

A cofactor for HIV-1 (human immunodeficiency virus-type 1) fusion and entry was identified with the use of a novel functional complementary DNA (cDNA) cloning strategy. This protein, designated "fusin," is a putative G protein-coupled receptor with seven transmembrane segments. Recombinant fusin enabled CD4-expressing nonhuman cell types to support HIV-1 Env-mediated cell fusion and HIV-1 infection. Antibodies to fusin blocked cell fusion and infection with normal CD4-positive human target cells. Fusin messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Fusin acted preferentially for T cell line-tropic isolates, in comparison to its activity with macrophagetropic HIV-1 isolates.

MeSH Terms

3T3 Cells Amino Acid Sequence Animals CD4 Antigens/physiology CD4-Positive T-Lymphocytes/virology Cell Line Cell Membrane/virology Chemokines/physiology Cloning, Molecular DNA, Complementary/genetics Disease Models, Animal GTP-Binding Proteins/metabolism Giant Cells HIV Envelope Protein gp120/physiology HIV-1/pathogenicity,physiology HeLa Cells Humans Leukocytes, Mononuclear/virology Macrophages/virology Membrane Fusion Membrane Proteins/genetics,physiology Mice Molecular Sequence Data RNA, Messenger/metabolism Receptors, CXCR4 Recombinant Proteins Transfection

Chemicals

CD4 Antigens Chemokines DNA, Complementary HIV Envelope Protein gp120 Membrane Proteins RNA, Messenger Receptors, CXCR4 Recombinant Proteins GTP-Binding Proteins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Feng Y

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases (NIAID), NIH, Bethesda, MD 20892, USA.

Broder C C

Kennedy P E

Berger E A

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.272.5263.872

Published

1996-05-10

Pages

872-7

Language

English

Region

United States

NLM ID

0404511

Subset

Corrections

CommentIn

PMID: 8629006

External Links

PubMed source DOI: 10.1126/science.272.5263.872 View journal details

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HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor.

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