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PMID: 8633070 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

ERK6, a mitogen-activated protein kinase involved in C2C12 myoblast differentiation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 9 ·1996-04-30 ·Pages 4355-9

Lechner C, Zahalka MA, Giot JF, Møller NP, Ullrich A

Abstract

ERK6, a mitogen-activated protein (MAP) kinase-related serine/threonine kinase, is highly expressed in human skeletal muscle and appears to function as a signal transducer during differentiation of myoblasts to myotubes. In transfected 293 cells, activation of the 45-kDa enzyme results in tyrosine-phosphorylated 46- and 56-kDa forms, which phosphorylate myelin basic protein. Overexpression of wild-type ERK6 or the inactive mutant Y185F has no effect on fibroblast and myoblast proliferation, but it enhances or inhibits C2C12 cell differentiation to myotubes, respectively. Our findings suggest ERK6 to be a tissue-specific, differentiation signal-transducing factor that is connected to phosphotyrosine-mediated signaling pathways distinct from those activating other members of the MAP kinase family such as LRK1 and ERK2.

MeSH Terms
3T3 Cells Amino Acid Sequence Animals Base Sequence Cell Differentiation Cloning, Molecular Enzyme Activation Gene Library Genetic Vectors Humans Insulin/pharmacology Kidney Mice Mitogen-Activated Protein Kinase 12 Mitogen-Activated Protein Kinases Molecular Sequence Data Muscle, Skeletal/enzymology Mutagenesis, Site-Directed Myelin Basic Protein/metabolism Oligodeoxyribonucleotides Phosphotyrosine/analysis Point Mutation Protein Kinases/biosynthesis,metabolism Recombinant Proteins/biosynthesis,metabolism Retroviridae Sequence Homology, Amino Acid Signal Transduction Transfection
Chemicals
Insulin Myelin Basic Protein Oligodeoxyribonucleotides Recombinant Proteins Phosphotyrosine Protein Kinases Mitogen-Activated Protein Kinase 12 Mitogen-Activated Protein Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lechner C
Department of Molecular Biology, Max-Planck-Institut für Biochemie, Martinsried, Germany.
Zahalka M A
Giot J F
Møller N P
Ullrich A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.9.4355
Published
1996-04-30
Pages
4355-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC39541
Subset
IM
Databases
GENBANK
X79483
External Links
PubMed source DOI: 10.1073/pnas.93.9.4355 PMC Full Text View journal details
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