Solid-phase radioimmunoassay for the detection of rotavirus.

Infection and immunity ·Vol. 16 ·No. 2 ·1977-05-00 ·Pages 439-44

Middleton PJ, Holdaway MD, Petric M, Szymanski MT, Tam JS

Abstract

A solid-phase radioimmunoassay method has been developed for the detection of rotavirus in the form of a purified antigen and in stool. The parameters of the radioimmunoassay were examined and optimized to give high sensitivity and same-day results. Compared with electron microscopy, the assay is up to 10 times as sensitive for detection of the virus in stool and up to 128 times as sensitive for detection of a purified virus antigen. In a field study on stool specimens it was at least as efficient as electron microscopy.

MeSH Terms

Antibodies, Viral/analysis Antigens, Viral/analysis Feces/microbiology Gastroenteritis/immunology Humans Neutralization Tests Radioimmunoassay/methods Reoviridae/immunology,isolation & purification

Chemicals

Antibodies, Viral Antigens, Viral

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Middleton P J

Holdaway M D

Petric M

Szymanski M T

Tam J S

References (11)

11 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.16.2.439-444.1977

Published

1977-05-00

Pages

439-44

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC420969

Subset

External Links

PubMed source DOI: 10.1128/iai.16.2.439-444.1977 PMC Full Text View journal details

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