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PMID: 8636036 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A complex four-gene operon containing essential cell division gene pbpB in Bacillus subtilis.

Journal of bacteriology ·Vol. 178 ·No. 8 ·1996-04-00 ·Pages 2343-50

Daniel RA, Williams AM, Errington J

Abstract

We have cloned and sequenced the promoter-proximal region of the Bacillus subtilis operon containing the pbpB gene, encoding essential penicillin-binding protein PBP2B. The first two genes in the operon, designated yllB and yllC, are significantly similar to genes of unknown function similarly positioned upstream of pbpB in Escherichia coli. Both B. subtilis genes are shown to be nonessential. The third B. subtilis gene, yllD, is essential, as is the correspondingly positioned ftsL gene of E. coli. The predicted product of yllD is similar to FtsL in size and distribution of charged residues but is not significantly related in primary amino acid sequence. The major promoter for the cluster lies upstream of the first gene, yllB, but at least one minor promoter lies within the yllC gene. The operon is transcribed throughout growth at a low level.

MeSH Terms
Amino Acid Sequence Bacillus subtilis/genetics,growth & development Bacterial Proteins/genetics Base Sequence Carrier Proteins/genetics Cell Cycle Proteins Cell Division Cloning, Molecular Escherichia coli/genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial Genes, Bacterial Hexosyltransferases Membrane Proteins/genetics Molecular Sequence Data Muramoylpentapeptide Carboxypeptidase/genetics Operon Penicillin-Binding Proteins Peptidoglycan Glycosyltransferase Peptidyl Transferases Promoter Regions, Genetic Sequence Analysis, DNA Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
Bacterial Proteins Carrier Proteins Cell Cycle Proteins Escherichia coli Proteins FtsI protein, E coli Membrane Proteins Penicillin-Binding Proteins YllB protein, Bacillus subtilis YllC protein, Bacillus subtilis YllD protein, Bacillus subtilis ftsL protein, E coli Peptidyl Transferases Hexosyltransferases Peptidoglycan Glycosyltransferase Muramoylpentapeptide Carboxypeptidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Daniel R A
Sir William Dunn School of Pathology, University of Oxford, United Kingdom.
Williams A M
Errington J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.8.2343-2350.1996
Published
1996-04-00
Pages
2343-50
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177943
Subset
IM
Databases
GENBANK
Z68230
External Links
PubMed source DOI: 10.1128/jb.178.8.2343-2350.1996 PMC Full Text View journal details
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