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PMID: 8636235 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A purified Drosophila septin complex forms filaments and exhibits GTPase activity.

The Journal of cell biology ·Vol. 133 ·No. 3 ·1996-05-00 ·Pages 605-16

Field CM, al-Awar O, Rosenblatt J, Wong ML, Alberts B, Mitchison TJ

Abstract

Septin proteins are necessary for cytokinesis in budding yeast and Drosophila and are thought to be the subunits of the yeast neck filaments. To test whether septins actually form filaments, an immunoaffinity approach was used to isolate a septin complex from Drosophila embryos. The purified complex is comprised of the three previously identified septin polypeptides Pnut, Sep2, and Sep1. Hydrodynamic and sequence data suggest that the complex is composed of a heterotrimer of homodimers. The complex copurifies with one molecule of bound guanine nucleotide per septin polypeptide. It binds and hydrolyzes exogenously added GTP. These observations together with conserved sequence motifs identify the septins as members of the GTPase superfamily. We discuss a model of filament structure and speculate as to how the filaments are organized within cells.

MeSH Terms
Actin Cytoskeleton/chemistry,metabolism,ultrastructure Amino Acid Sequence Animals Blotting, Western Deoxyribonucleases/isolation & purification,metabolism Drosophila/enzymology Drosophila Proteins Exoribonucleases Fungal Proteins/isolation & purification,metabolism GTP Phosphohydrolases/metabolism Guanosine Triphosphate/metabolism Hydrolysis Microfilament Proteins Microscopy, Electron Molecular Sequence Data Proteins/isolation & purification,metabolism Saccharomyces cerevisiae Proteins
Chemicals
Drosophila Proteins Fungal Proteins Microfilament Proteins Proteins Saccharomyces cerevisiae Proteins pnut protein, Drosophila Guanosine Triphosphate Deoxyribonucleases Exoribonucleases XRN1 protein, S cerevisiae GTP Phosphohydrolases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Field C M
Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448, USA. [email protected]
al-Awar O
Rosenblatt J
Wong M L
Alberts B
Mitchison T J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.133.3.605
Email
[email protected]
Published
1996-05-00
Pages
605-16
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120824
Subset
IM
Grants
NIGMS NIH HHS · GM 23928 · United States
NIGMS NIH HHS · GM50868 · United States
External Links
PubMed source DOI: 10.1083/jcb.133.3.605 PMC Full Text View journal details
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