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PMID: 8641269 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.

The EMBO journal ·Vol. 15 ·No. 9 ·1996-05-01 ·Pages 2031-49

Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L

Abstract

The complete nucleotide sequence of Saccharomyces cerevisiae chromosome X (745 442 bp) reveals a total of 379 open reading frames (ORFs), the coding region covering approximately 75% of the entire sequence. One hundred and eighteen ORFs (31%) correspond to genes previously identified in S. cerevisiae. All other ORFs represent novel putative yeast genes, whose function will have to be determined experimentally. However, 57 of the latter subset (another 15% of the total) encode proteins that show significant analogy to proteins of known function from yeast or other organisms. The remaining ORFs, exhibiting no significant similarity to any known sequence, amount to 54% of the total. General features of chromosome X are also reported, with emphasis on the nucleotide frequency distribution in the environment of the ATG and stop codons, the possible coding capacity of at least some of the small ORFs (<100 codons) and the significance of 46 non-canonical or unpaired nucleotides in the stems of some of the 24 tRNA genes recognized on this chromosome.

MeSH Terms
Amino Acid Sequence Base Sequence Centromere Chromosome Mapping Chromosomes, Fungal Codon, Terminator Molecular Sequence Data Multigene Family Open Reading Frames RNA, Transfer/chemistry Saccharomyces cerevisiae/genetics Telomere
Chemicals
Codon, Terminator RNA, Transfer
Authors & Affiliations
25 authors, click to expand affiliations / ORCID
Galibert F
CNRS Recombinaisons Génétiques, Faculté de Médecine, Rennes, France.
Alexandraki D
Baur A
Boles E
Chalwatzis N
Chuat J C
Coster F
Cziepluch C
De Haan M
Domdey H
Durand P
Entian K D
Gatius M
Goffeau A
Grivell L A
Hennemann A
Herbert C J
Heumann K
Hilger F
Hollenberg C P
Huang M E
Jacq C
Jauniaux J C
Katsoulou C
Karpfinger-Hartl L
References (41)
41 references, click to expand
  1. Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli.
    Nature. 1979 Oct 25;281(5733):646-50 PMID: 399327
  2. Editing corrects mispairing in the acceptor stem of bean and potato mitochondrial phenylalanine transfer RNAs.
    Nucleic Acids Res. 1993 Oct 25;21(21):4909-14 PMID: 8177739
  3. Functional selection and analysis of yeast centromeric DNA.
    Cell. 1985 Oct;42(3):913-21 PMID: 2996783
  4. The codon Adaptation Index--a measure of directional synonymous codon usage bias, and its potential applications.
    Nucleic Acids Res. 1987 Feb 11;15(3):1281-95 PMID: 3547335
  5. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
    Nucleic Acids Res. 1987 Oct 26;15(20):8125-48 PMID: 3313277
  6. Improved tools for biological sequence comparison.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 PMID: 3162770
  7. Site-specific DNA endonuclease and RNA maturase activities of two homologous intron-encoded proteins from yeast mitochondria.
    Cell. 1989 Feb 10;56(3):431-41 PMID: 2536593
  8. Double-strand breaks at an initiation site for meiotic gene conversion.
    Nature. 1989 Mar 2;338(6210):87-90 PMID: 2645528
  9. Identifying potential tRNA genes in genomic DNA sequences.
    J Mol Biol. 1991 Aug 5;220(3):659-71 PMID: 1870126
  10. The MAT locus revisited within a 9.8 kb fragment of chromosome III containing BUD5 and two new open reading frames.
    Yeast. 1991 Nov;7(8):881-8 PMID: 1789011
  11. Purification and complete sequence of a small proteolipid associated with the plasma membrane H(+)-ATPase of Saccharomyces cerevisiae.
    J Biol Chem. 1992 Mar 25;267(9):6425-8 PMID: 1532582
  12. Evolutionarily recent transfer of a group I mitochondrial intron to telomere regions in Saccharomyces cerevisiae.
    Curr Genet. 1991 Nov;20(5):411-5 PMID: 1807832
  13. The complete DNA sequence of yeast chromosome III.
    Nature. 1992 May 7;357(6373):38-46 PMID: 1574125
  14. CODONS: a microcomputer program for codon usage analysis.
    J Hered. 1992 May-Jun;83(3):239-40 PMID: 1624775
  15. Additive, cooperative and anti-cooperative effects between identity nucleotides of a tRNA.
    EMBO J. 1993 Jul;12(7):2949-57 PMID: 8335008
  16. A region of the C-terminal part of the 11-kDa subunit of ubiquinol-cytochrome-c oxidoreductase of the yeast Saccharomyces cerevisiae contributes to the structure of the Qout reaction domain.
    Eur J Biochem. 1993 Aug 1;215(3):601-9 PMID: 8394810
  17. Complete DNA sequence of yeast chromosome XI.
    Nature. 1994 Jun 2;369(6479):371-8 PMID: 8196765
  18. The chromosome end in yeast: its mosaic nature and influence on recombinational dynamics.
    Genetics. 1994 Mar;136(3):789-802 PMID: 8005434
  19. Two distinct genes encode small isoproteolipids affecting plasma membrane H(+)-ATPase activity of Saccharomyces cerevisiae.
    J Biol Chem. 1994 Aug 19;269(33):21262-8 PMID: 8063750
  20. Approaching the function of new genes by detection of their potential upstream activation sequences in Saccharomyces cerevisiae: application to chromosome III.
    Curr Genet. 1994 May;25(5):396-406 PMID: 8082184
  21. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
    Science. 1994 Sep 30;265(5181):2077-82 PMID: 8091229
  22. A voltage-gated chloride channel in the yeast Saccharomyces cerevisiae.
    J Mol Biol. 1994 Sep 30;242(4):595-8 PMID: 7932715
  23. Revised nucleotide sequence of the COR region of yeast Saccharomyces cerevisiae chromosome X.
    Yeast. 1994 Jun;10(6):811-8 PMID: 7975898
  24. Sequence and function analysis of a 9.46 kb fragment of Saccharomyces cerevisiae chromosome X.
    Yeast. 1994 Jul;10(7):965-73 PMID: 7985424
  25. Construction of a cosmid contig and of an EcoRI restriction map of yeast chromosome X.
    DNA Seq. 1994;4(5):293-300 PMID: 7803814
  26. Complete DNA sequence of yeast chromosome II.
    EMBO J. 1994 Dec 15;13(24):5795-809 PMID: 7813418
  27. Sequence and function analysis of a 9.74 kb fragment of Saccharomyces cerevisiae chromosome X including the BCK1 gene.
    Yeast. 1994 Nov;10(11):1481-8 PMID: 7871887
  28. A complete set of marked telomeres in Saccharomyces cerevisiae for physical mapping and cloning.
    Genetics. 1995 Jan;139(1):125-36 PMID: 7705618
  29. Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X.
    Yeast. 1994 Dec;10(12):1657-62 PMID: 7725802
  30. The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3809-13 PMID: 7731988
  31. The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes.
    Yeast. 1994 Sep;10(9):1235-49 PMID: 7754713
  32. Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C.
    Yeast. 1995 Jan;11(1):53-5 PMID: 7762301
  33. Sequence of a 17.1 kb DNA fragment from chromosome X of Saccharomyces cerevisiae includes the mitochondrial ribosomal protein L8.
    Yeast. 1995 Jan;11(1):57-60 PMID: 7762302
  34. Sequence analysis of the right end of chromosome XV in Saccharomyces cerevisiae: an insight into the structural and functional significance of sub-telomeric repeat sequences.
    Yeast. 1995 Apr 15;11(4):371-82 PMID: 7785338
  35. A new family of outwardly rectifying potassium channel proteins with two pore domains in tandem.
    Nature. 1995 Aug 24;376(6542):690-5 PMID: 7651518
  36. Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases.
    Yeast. 1995 Jun 30;11(8):775-81 PMID: 7668047
  37. Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
    Nat Genet. 1995 Jul;10(3):261-8 PMID: 7670463
  38. A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg).
    Yeast. 1995 Jul;11(9):873-83 PMID: 7483851
  39. Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site.
    Yeast. 1995 Jun 15;11(7):681-9 PMID: 7483841
  40. The sequence of 24.3 kb from chromosome X reveals five complete open reading frames, all of which correspond to new genes, and a tandem insertion of a Ty1 transposon.
    Yeast. 1995 Sep 30;11(12):1179-86 PMID: 8619316
  41. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-05-01
Pages
2031-49
Language
English
Region
England
NLM ID
8208664
PMCID
PMC450125
Subset
IM
Grants
Wellcome Trust · United Kingdom
Databases
GENBANK
U17709, X01963
PIR
45153, A23082, A39736, C37281, S05829, S05888, S16693, S23553, S23891, S25329, S26706, S27422, S38012, S40400, S45157, S45159, S45161, S45870, S46033, S46183, S46633, S46638, S46640, S46641, S46643, S48516
SWISSPROT
P35123, PO7464
External Links
PubMed source PMC Full Text View journal details
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