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PMID: 8641759 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sporadic invasion of cultured epithelial cells by Haemophilus influenzae type b.

Infection and immunity ·Vol. 64 ·No. 3 ·1996-03-00 ·Pages 1051-3

Johnson PD, Oppedisano F, Bennett-Wood V, Gilbert GL, Robins-Browne RM

Abstract

While working with an in vitro invasion assay, we observed that Haemophilus influenzae type b occasionally exhibits highly invasive behavior. The phenomenon is not inhibited by colchicine or cytochalasin but is dependent on the presence of supplemental CO2. We propose that sporadic invasiveness may correlate with the unknown events that precede Haemophilus influenzae type b bacteremia.

MeSH Terms
Bacterial Adhesion Carbon Dioxide/pharmacology Cells, Cultured Colchicine/pharmacology Epithelium/microbiology Haemophilus influenzae/physiology Humans
Chemicals
Carbon Dioxide Colchicine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Johnson P D
Department of Microbiology and Infectious Diseases, Royal Children's Hospital, Parkville, Victoria, Australia.
Oppedisano F
Bennett-Wood V
Gilbert G L
Robins-Browne R M
References (20)
20 references, click to expand
  1. Quantitative assessment of the ability of Escherichia coli to invade cultured animal cells.
    Microb Pathog. 1992 Feb;12(2):159-64 PMID: 1584008
  2. Rifampicin prophylaxis for throat carriage of Haemophilus influenzae type b in patients with invasive disease and their contacts.
    BMJ. 1991 Jun 15;302(6790):1432-5 PMID: 2070109
  3. Outer membrane protein subtypes of Haemophilus influenzae type b isolates causing invasive disease in Victoria, Australia, from 1988 to 1990.
    J Clin Microbiol. 1992 Jul;30(7):1879-81 PMID: 1629347
  4. The gal locus from Haemophilus influenzae: cloning, sequencing and the use of gal mutants to study lipopolysaccharide.
    Mol Microbiol. 1992 Oct;6(20):3051-63 PMID: 1282642
  5. Antibodies to Haemophilus influenzae type b outer membrane proteins in children with epiglottitis or meningitis and in healthy controls.
    Infect Immun. 1993 Apr;61(4):1531-7 PMID: 8454359
  6. Identification of a new locus involved in expression of Haemophilus influenzae type b lipooligosaccharide.
    Infect Immun. 1994 Nov;62(11):4861-7 PMID: 7523298
  7. Reassessment of the number of auxiliary genes essential for expression of high-level methicillin resistance in Staphylococcus aureus.
    Antimicrob Agents Chemother. 1994 Nov;38(11):2590-8 PMID: 7872753
  8. Human microvascular endothelial tissue culture cell model for studying pathogenesis of Brazilian purpuric fever.
    Infect Immun. 1995 Jun;63(6):2317-22 PMID: 7768615
  9. Carbon dioxide regulated secretion of the EaeB protein of enteropathogenic Escherichia coli.
    FEMS Microbiol Lett. 1995 Jun 1;129(1):63-7 PMID: 7781991
  10. Clinical manifestations and outcome of Haemophilus influenzae type b disease.
    J Paediatr Child Health. 1995 Apr;31(2):99-104 PMID: 7794633
  11. Human serum activities against Hemophilus influenzae, type b.
    J Clin Invest. 1972 Jan;51(1):31-8 PMID: 4536614
  12. Interaction of magnesium ion, oxygen tension, and temperature in the production of toxic-shock-syndrome toxin-1 by Staphylococcus aureus.
    J Infect Dis. 1987 Apr;155(4):812-5 PMID: 3102633
  13. Comparison of the ability of enteroinvasive Escherichia coli, Salmonella typhimurium, Yersinia pseudotuberculosis, and Yersinia enterocolitica to enter and replicate within HEp-2 cells.
    Infect Immun. 1987 Jul;55(7):1674-9 PMID: 3298064
  14. Identification of invasin: a protein that allows enteric bacteria to penetrate cultured mammalian cells.
    Cell. 1987 Aug 28;50(5):769-78 PMID: 3304658
  15. Cloning and CO2-dependent expression of the genetic region for encapsulation from Bacillus anthracis.
    Mol Microbiol. 1988 May;2(3):371-6 PMID: 2456447
  16. Adhesion to and invasion of HEp-2 cells by Campylobacter spp.
    Infect Immun. 1989 Oct;57(10):2984-90 PMID: 2550368
  17. Phase-variable lipopolysaccharide structures enhance the invasive capacity of Haemophilus influenzae.
    Infect Immun. 1990 Oct;58(10):3455-7 PMID: 2401571
  18. Penetration and replication of Edwardsiella spp. in HEp-2 cells.
    Infect Immun. 1991 Jan;59(1):154-61 PMID: 1987028
  19. Loss of capsule expression by Haemophilus influenzae type b results in enhanced adherence to and invasion of human cells.
    Infect Immun. 1991 Apr;59(4):1325-33 PMID: 1672302
  20. Molecular biology of phase-variable lipopolysaccharide biosynthesis by Haemophilus influenzae.
    J Infect Dis. 1992 Jun;165 Suppl 1:S90-2 PMID: 1588188
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.64.3.1051-1053.1996
Published
1996-03-00
Pages
1051-3
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC173880
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.64.3.1051-1053.1996 PMC Full Text View journal details
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