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PMID: 8642293 Published · ppublish English Comment Comparative Study Journal Article Research Support, Non-U.S. Gov't

B cells solicit their own help from T cells.

The Journal of experimental medicine ·Vol. 183 ·No. 3 ·1996-03-01 ·Pages 891-9

Stockinger B, Zal T, Zal A, Gray D

Abstract

We have made use of T cell receptor (TCR)-transgenic mice with CD4+ T cells expressing a receptor specific for the self-antigen C5 (fifth component of complement) to study the role of different antigen-presenting cells in the determination of CD4+ T cell effector type. Contact of T cells from C5 TCR-transgenic mice with C5 protein or C5 peptide in vivo or in vitro induces biased T helper cell (Th) 1 type responses resulting in exclusive production of high levels of interferon gamma and interleukin (IL) 2. Transgenic mice, in contrast to nontransgenic littermates, do not generate an antibody response to C5. We show in this paper that B cell presentation in vitro induces a switch to the Th2 subset indicated by production of IL-4, and targetting C5 to B cells in vivo results in the generation of C5-specific antibodies.

MeSH Terms
Animals Antibody Formation Antigen-Presenting Cells/immunology B-Lymphocytes/immunology CD4-Positive T-Lymphocytes/immunology Cell Line Complement C5/immunology Hybridomas Immunoglobulin G/biosynthesis,classification Immunoglobulin M/biosynthesis Interferon-gamma/biosynthesis Interleukin-2/biosynthesis Lymphocyte Activation Lymphoma Mice Mice, Inbred A Mice, Transgenic Receptors, Antigen, T-Cell/biosynthesis,genetics,immunology Signal Transduction Species Specificity T-Lymphocytes/immunology T-Lymphocytes, Helper-Inducer/immunology Thymus Gland/immunology
Chemicals
Complement C5 Immunoglobulin G Immunoglobulin M Interleukin-2 Receptors, Antigen, T-Cell Interferon-gamma
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stockinger B
National Institute for Medical Research, Division of Molecular Immunology, London, United Kingdom.
Zal T
Zal A
Gray D
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.183.3.891
Published
1996-03-01
Pages
891-9
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2192359
Subset
IM
Corrections
CommentOn
PMID: 8642274
External Links
PubMed source DOI: 10.1084/jem.183.3.891 PMC Full Text View journal details
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